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Int. J. Mol. Sci. 2009, 10(5), 2136-2145; doi:10.3390/ijms10052136
Article

The Effect of Self-Assembling Peptide RADA16-I on the Growth of Human Leukemia Cells in Vitro and in Nude Mice

1
,
1
,
1
,
1
 and
1, 2, 3,*
1 Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, Chengdu, 610041, China 2 West China Medical School, West China Hospital, Sichuan University, Guo Xue Xiang 37, Chengdu, 610041, Chengdu, China 3 Center for Biomedical Engineering, NE47-379, Massachusetts Institute of Technology, Cambridge, MA 02139-4307, USA
* Author to whom correspondence should be addressed.
Received: 31 March 2009 / Revised: 30 April 2009 / Accepted: 12 May 2009 / Published: 14 May 2009
(This article belongs to the Special Issue Molecular Self-Assembly)
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Abstract

Nanofiber scaffolds formed by self-assembling peptide RADA16-I have been used for the study of cell proliferation to mimic an extracellular matrix. In this study, we investigated the effect of RADA16-I on the growth of human leukemia cells in vitro and in nude mice. Self-assembly assessment showed that RADA16-I molecules have excellent self-assembling ability to form stable nanofibers. MTT assay displayed that RADA16-I has no cytotoxicity for leukemia cells and human umbilical vein endothelial cells (HUVECs) in vitro. However, RADA16-I inhibited the growth of K562 tumors in nude mice. Furthermore, we found RADA16-I inhibited vascular tube-formation by HUVECs in vitro. Our data suggested that nanofiber scaffolds formed by RADA16-I could change tumor microenvironments, and inhibit the growth of tumors. The study helps to encourage further design of self-assembling systems for cancer therapy.
Keywords: self-assembling peptide RADA16-I; nanofiber scaffolds; mimic extracellular matrix; tumor microenvironments; cancer therapy self-assembling peptide RADA16-I; nanofiber scaffolds; mimic extracellular matrix; tumor microenvironments; cancer therapy
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Tang, C.; Shao, X.; Sun, B.; Huang, W.; Zhao, X. The Effect of Self-Assembling Peptide RADA16-I on the Growth of Human Leukemia Cells in Vitro and in Nude Mice. Int. J. Mol. Sci. 2009, 10, 2136-2145.

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