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The Effect of Self-Assembling Peptide RADA16-I on the Growth of Human Leukemia Cells in Vitro and in Nude Mice
Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, Chengdu, 610041, China
West China Medical School, West China Hospital, Sichuan University, Guo Xue Xiang 37, Chengdu, 610041, Chengdu, China
Center for Biomedical Engineering, NE47-379, Massachusetts Institute of Technology, Cambridge, MA 02139-4307, USA
* Author to whom correspondence should be addressed.
Received: 31 March 2009; in revised form: 30 April 2009 / Accepted: 12 May 2009 / Published: 14 May 2009
Abstract: Nanofiber scaffolds formed by self-assembling peptide RADA16-I have been used for the study of cell proliferation to mimic an extracellular matrix. In this study, we investigated the effect of RADA16-I on the growth of human leukemia cells in vitro and in nude mice. Self-assembly assessment showed that RADA16-I molecules have excellent self-assembling ability to form stable nanofibers. MTT assay displayed that RADA16-I has no cytotoxicity for leukemia cells and human umbilical vein endothelial cells (HUVECs) in vitro. However, RADA16-I inhibited the growth of K562 tumors in nude mice. Furthermore, we found RADA16-I inhibited vascular tube-formation by HUVECs in vitro. Our data suggested that nanofiber scaffolds formed by RADA16-I could change tumor microenvironments, and inhibit the growth of tumors. The study helps to encourage further design of self-assembling systems for cancer therapy.
Keywords: self-assembling peptide RADA16-I; nanofiber scaffolds; mimic extracellular matrix; tumor microenvironments; cancer therapy
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MDPI and ACS Style
Tang, C.; Shao, X.; Sun, B.; Huang, W.; Zhao, X. The Effect of Self-Assembling Peptide RADA16-I on the Growth of Human Leukemia Cells in Vitro and in Nude Mice. Int. J. Mol. Sci. 2009, 10, 2136-2145.
Tang C, Shao X, Sun B, Huang W, Zhao X. The Effect of Self-Assembling Peptide RADA16-I on the Growth of Human Leukemia Cells in Vitro and in Nude Mice. International Journal of Molecular Sciences. 2009; 10(5):2136-2145.
Tang, Chengkang; Shao, Ximing; Sun, Binbin; Huang, Wenli; Zhao, Xiaojun. 2009. "The Effect of Self-Assembling Peptide RADA16-I on the Growth of Human Leukemia Cells in Vitro and in Nude Mice." Int. J. Mol. Sci. 10, no. 5: 2136-2145.