Protein GB1 Folding and Assembly from Structural Elements
Abstract
:1. Introduction
2. Results and Discussion
2.1. Salt dependence of CD spectra for fragment mixtures
2.2. Salt-dependent residual structure in C16
2.3. Temperature dependence of CD spectra for fragment mixtures
2.4. Affinity between N41-QDD and C16
2.5. Prefolding of C16 favors complex formation
2.6. Comparable salt effects on assembly and folding equilibria
3. Experimental Section
3.1. Cloning, expression and purification of PGB1 fragments
3.2. Expression and purification of intact PGB1-QDD
3.3. Circular dichroism spectroscopy
3.4. 1H-NMR spectroscopy
3.5. Binding model
3.6. Data Analysis
4. Conclusions
Acknowledgments
References and Notes
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[NaCl] | logKA at 5 °C | logKA at 25 °C | logKA.Eff at 5 °C | logKA.Eff at 25 °C |
---|---|---|---|---|
no added | 5.4 ± 0.4 | 4.3 ± 0.2 | 5.4 ± 0.3 | 4.2 ± 0.2 |
150 mM | 4.3 ± 0.1 | 3.7 ± 0.1 | 4.3 ± 0.1 | 3.6 ± 0.1 |
500 mM | 4.4 ± 0.2 | 3.4 ± 0.2 | 4.3 ± 0.2 | 3.4 ± 0.1 |
2000 mM | 6.0 ± 0.8 | 4.9 ± 0.4 | 5.7 ± 0.6 | 4.8 ± 0.4 |
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Bauer, M.C.; Xue, W.-F.; Linse, S. Protein GB1 Folding and Assembly from Structural Elements. Int. J. Mol. Sci. 2009, 10, 1552-1566. https://doi.org/10.3390/ijms10041552
Bauer MC, Xue W-F, Linse S. Protein GB1 Folding and Assembly from Structural Elements. International Journal of Molecular Sciences. 2009; 10(4):1552-1566. https://doi.org/10.3390/ijms10041552
Chicago/Turabian StyleBauer, Mikael C., Wei-Feng Xue, and Sara Linse. 2009. "Protein GB1 Folding and Assembly from Structural Elements" International Journal of Molecular Sciences 10, no. 4: 1552-1566. https://doi.org/10.3390/ijms10041552