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Expression of Mipu1 in Response to Myocardial Infarction in Rats
Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, 410008, Hunan, People’s Republic of China
Department of Digestive Internal Medicine, People’ Hospital of Pingxiang City, 128 Guangchang Road, Pingxiang, 337055, Jiangxi, People’s Republic of China
Department of Rheumatology and Clinical Immunology, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, 410008, Hunan, People’s Republic of China
* Author to whom correspondence should be addressed.
Received: 13 October 2008; in revised form: 20 November 2008 / Accepted: 1 December 2008 / Published: 3 February 2009
Abstract: Myocardial ischemic preconditioning up-regulated protein 1 (Mipu1) was cloned in our laboratory. Male Wistar rats were subjected to left anterior coronary artery ligation and sham-operation and sacrificed at 1 h, 3 h, 6 h, 12 h, 24 h, 3 d or 5 d after ligation. Expression of Mipu1 mRNA and protein were assessed by Northern blotting, real-time quantitative RT-PCR, In Situ hybridization and Western blotting. Expression of Mipu1 was up-regulated at 3 h and lasted to 12 h with a peak at 6 h. Mipu1 mRNA and protein signals express in the endothelium and myocardium in normal and infarcted heart, mainly in infarcted zone. Fluorescent immunocytochemistry showed that Mipu1 protein was localized to the nuclei of H9c2 myogenic cells and was upregulated after the cells being exposed to H2O2. These observations indicates that Mipu1 may play a role in maintaining vascular homeostasis and protecting the myogenic cells from being injured by ischemia-reperfusion or oxidation stress.
Keywords: Mipu1; Myocardial ischemia; Gene expression; Real-time RT-PCR
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MDPI and ACS Style
Wang, G.; Zuo, X.; Liu, J.; Jiang, L.; Liu, Y.; Zheng, Y.; Zhang, B.; Xiao, X. Expression of Mipu1 in Response to Myocardial Infarction in Rats. Int. J. Mol. Sci. 2009, 10, 492-506.
Wang G, Zuo X, Liu J, Jiang L, Liu Y, Zheng Y, Zhang B, Xiao X. Expression of Mipu1 in Response to Myocardial Infarction in Rats. International Journal of Molecular Sciences. 2009; 10(2):492-506.
Wang, Guiliang; Zuo, Xiaoxia; Liu, Junwen; Jiang, Lei; Liu, Ying; Zheng, Yazhu; Zhang, Bin; Xiao, Xianzhong. 2009. "Expression of Mipu1 in Response to Myocardial Infarction in Rats." Int. J. Mol. Sci. 10, no. 2: 492-506.