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Extraction of Teucrium manghuaense and Evaluation of the Bioactivity of Its Extract
Analysis and Test Center of Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Wanning 571533, China
* Author to whom correspondence should be addressed.
Received: 15 July 2009; in revised form: 9 September 2009 / Accepted: 24 September 2009 / Published: 9 October 2009
Abstract: The ethanol extract of Teucrium manghuaense grown in Hainan province (China) was analysed by GC and GC/MS. Of the constituents 84–96% were identified on the basis of their GC retention times and their mass spectra in regard to authentic compounds. The results revealed that it contained 9-methyl-9-azabicyclo[4.2.1]nonane (7.43%), 2-methylpyrrolidine (19.42%), 3,7,11,15-tetramethyl-2-hexadecen-1-ol (10.84%), and squalene (28.55%), as major components, constituting 66.24% of the extract. The chemical characterization of the ethanol extract by GC/MS also allowed identification of 1-octen-3-one (3.41%), 2-pentyl-piperidine (2.25%), 1-(2-methyl-1-propenyl)-piperidine (4.63%), 2,2'-diethoxy-5,5'-bi-1-pyrroline (0.41%), (Z,Z,Z)-9,12,15-octadecatrieniic acid, 2,3-dihydroxypropyl ester (1.56%), vitamin E (2.95%) and stigmasterol (1.39%). Finally, the antioxidant and anti-tumor activities of the ethanol extract have been evaluated. Results show that the extract of Teucrium manghuaense leaf possesses strong DPPH-, hydroxyl radical scavenging activity and anti-tumor activity.
Keywords: Teucrium manghuaense; extraction; squalene; GC-MS; antioxidant; anti-tumor
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Yin, G.; Zeng, H.; He, M.; Wang, M. Extraction of Teucrium manghuaense and Evaluation of the Bioactivity of Its Extract. Int. J. Mol. Sci. 2009, 10, 4330-4341.
Yin G, Zeng H, He M, Wang M. Extraction of Teucrium manghuaense and Evaluation of the Bioactivity of Its Extract. International Journal of Molecular Sciences. 2009; 10(10):4330-4341.
Yin, Guihao; Zeng, Huicai; He, Mingjun; Wang, Mingyue. 2009. "Extraction of Teucrium manghuaense and Evaluation of the Bioactivity of Its Extract." Int. J. Mol. Sci. 10, no. 10: 4330-4341.