Next Article in Journal
Previous Article in Journal
Int. J. Mol. Sci. 2009, 10(10), 4210-4222; doi:10.3390/ijms10104210
Article

Effects of Acrylamide on the Activity and Structure of Human Brain Creatine Kinase

1, 2,4, 3, 3, 2,3, 4,*  and 1,*
Received: 4 September 2009; in revised form: 16 September 2009 / Accepted: 24 September 2009 / Published: 28 September 2009
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [456 KB, uploaded 19 June 2014]
Abstract: Acrylamide is widely used worldwide in industry and it can also be produced by the cooking and processing of foods. It is harmful to human beings, and human brain CK (HBCK) has been proposed to be one of the important targets of acrylamide. In this research, we studied the effects of acrylamide on HBCK activity, structure and the potential binding sites. Compared to CKs from rabbit, HBCK was fully inactivated at several-fold lower concentrations of acrylamide, and exhibited distinct properties upon acrylamide-induced inactivation and structural changes. The binding sites of acrylamide were located at the cleft between the N- and C-terminal domains of CK, and Glu232 was one of the key binding residues. The effects of acrylamide on CK were proposed to be isoenzyme- and species-specific, and the underlying molecular mechanisms were discussed.
Keywords: acrylamide; human brain creatine kinase; inactivation; docking simulation acrylamide; human brain creatine kinase; inactivation; docking simulation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Sheng, Q.; Zou, H.-C.; Lü, Z.-R.; Zou, F.; Park, Y.-D.; Yan, Y.-B.; Yao, S.-J. Effects of Acrylamide on the Activity and Structure of Human Brain Creatine Kinase. Int. J. Mol. Sci. 2009, 10, 4210-4222.

AMA Style

Sheng Q, Zou H-C, Lü Z-R, Zou F, Park Y-D, Yan Y-B, Yao S-J. Effects of Acrylamide on the Activity and Structure of Human Brain Creatine Kinase. International Journal of Molecular Sciences. 2009; 10(10):4210-4222.

Chicago/Turabian Style

Sheng, Qing; Zou, He-Chang; Lü, Zhi-Rong; Zou, Fei; Park, Yong-Doo; Yan, Yong-Bin; Yao, Shan-Jing. 2009. "Effects of Acrylamide on the Activity and Structure of Human Brain Creatine Kinase." Int. J. Mol. Sci. 10, no. 10: 4210-4222.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert