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Int. J. Mol. Sci. 2009, 10(10), 4210-4222; doi:10.3390/ijms10104210
Article

Effects of Acrylamide on the Activity and Structure of Human Brain Creatine Kinase

1, 2,4, 3, 3, 2,3, 4,*  and 1,*
1 Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China 2 Yangtze Delta Region Institute of Tsinghua University, Jiaxing 314050, China 3 Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China 4 State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
* Authors to whom correspondence should be addressed.
Received: 4 September 2009 / Revised: 16 September 2009 / Accepted: 24 September 2009 / Published: 28 September 2009
(This article belongs to the Section Bioinorganic Chemistry)
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Abstract

Acrylamide is widely used worldwide in industry and it can also be produced by the cooking and processing of foods. It is harmful to human beings, and human brain CK (HBCK) has been proposed to be one of the important targets of acrylamide. In this research, we studied the effects of acrylamide on HBCK activity, structure and the potential binding sites. Compared to CKs from rabbit, HBCK was fully inactivated at several-fold lower concentrations of acrylamide, and exhibited distinct properties upon acrylamide-induced inactivation and structural changes. The binding sites of acrylamide were located at the cleft between the N- and C-terminal domains of CK, and Glu232 was one of the key binding residues. The effects of acrylamide on CK were proposed to be isoenzyme- and species-specific, and the underlying molecular mechanisms were discussed.
Keywords: acrylamide; human brain creatine kinase; inactivation; docking simulation acrylamide; human brain creatine kinase; inactivation; docking simulation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sheng, Q.; Zou, H.-C.; Lü, Z.-R.; Zou, F.; Park, Y.-D.; Yan, Y.-B.; Yao, S.-J. Effects of Acrylamide on the Activity and Structure of Human Brain Creatine Kinase. Int. J. Mol. Sci. 2009, 10, 4210-4222.

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