Effects of Acrylamide on the Activity and Structure of Human Brain Creatine Kinase
Abstract
:1. Introduction
2. Results and Discussion
2.1. Inactivation of HBCK by Acrylamide
2.2. Effect of Acrylamide on the Tertiary Structure of HBCK
2.3. Acrylamide Binding-Site on HBCK by Docking Simulation
3. Experimental Section
3.1. Materials and Protein Expression and Purification
3.2. HBCK Activity Assay
3.3. ANS Fluorescence
3.4. In silico Docking of HBCK and Acrylamide
4. Conclusions
Acknowledgments
References
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Acrylamide (mM) | Inactivation rate constants (10−3 s−1) | ΔΔG°′ (kJ/mol·min−1) | |
---|---|---|---|
k1 | k2 | ||
50 | 2.0 | 0.31 | 25.5 |
100 | 2.9 | 0.21 | 24.6 |
200 | 5.7 | 0.39 | 22.9 |
400 | 3.5 | 0.65 | 24.1 |
800 | 3.6 | 0.68 | 24.0 |
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Sheng, Q.; Zou, H.-C.; Lü, Z.-R.; Zou, F.; Park, Y.-D.; Yan, Y.-B.; Yao, S.-J. Effects of Acrylamide on the Activity and Structure of Human Brain Creatine Kinase. Int. J. Mol. Sci. 2009, 10, 4210-4222. https://doi.org/10.3390/ijms10104210
Sheng Q, Zou H-C, Lü Z-R, Zou F, Park Y-D, Yan Y-B, Yao S-J. Effects of Acrylamide on the Activity and Structure of Human Brain Creatine Kinase. International Journal of Molecular Sciences. 2009; 10(10):4210-4222. https://doi.org/10.3390/ijms10104210
Chicago/Turabian StyleSheng, Qing, He-Chang Zou, Zhi-Rong Lü, Fei Zou, Yong-Doo Park, Yong-Bin Yan, and Shan-Jing Yao. 2009. "Effects of Acrylamide on the Activity and Structure of Human Brain Creatine Kinase" International Journal of Molecular Sciences 10, no. 10: 4210-4222. https://doi.org/10.3390/ijms10104210