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Int. J. Mol. Sci. 2009, 10(1), 345-353; doi:10.3390/ijms10010345
Article

Exochelin Production in Mycobacterium neoaurum

1,2
Received: 26 September 2008; in revised form: 13 January 2009 / Accepted: 15 January 2009 / Published: 20 January 2009
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37ºC within 3 – 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most mycobacteria produce siderophores. One example is exochelin, which is extracellular and water-soluble. In this report, the production of exochelin in M. neoaurum was induced in iron-deficiency, but repressed under iron-sufficiency growth conditions. It is however not induced under zinc-deficiency growth conditions. The growth of this mycobacterium was correlated with exochelin secretion under iron-deficiency culture conditions. When M. neoaurum was grown in defined medium containing 0.04 μg Fe(III)/mL (final concentration), the production of exochelin reached a maximum and the corresponding cell growth was comparable to that under iron-sufficiency conditions. In this study, exochelin was purified from spent supernatant of M. neoaurum bysemi-preparative chromatography. When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed. It is proposed that iron uptake in M. neoaurum is exochelin-mediated.
Keywords: Mycobacterium neoaurum; siderophore; exochelin; iron-deficiency; ferri-exochelin; zinc-deficiency Mycobacterium neoaurum; siderophore; exochelin; iron-deficiency; ferri-exochelin; zinc-deficiency
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chan, K.-G. Exochelin Production in Mycobacterium neoaurum. Int. J. Mol. Sci. 2009, 10, 345-353.

AMA Style

Chan K-G. Exochelin Production in Mycobacterium neoaurum. International Journal of Molecular Sciences. 2009; 10(1):345-353.

Chicago/Turabian Style

Chan, Kok-Gan. 2009. "Exochelin Production in Mycobacterium neoaurum." Int. J. Mol. Sci. 10, no. 1: 345-353.



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