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Exochelin Production in Mycobacterium neoaurum
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
Centre for Research in Biotechnology for Agriculture (CEBAR), Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia
Received: 26 September 2008; in revised form: 13 January 2009 / Accepted: 15 January 2009 / Published: 20 January 2009
Abstract: Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37ºC within 3 – 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most mycobacteria produce siderophores. One example is exochelin, which is extracellular and water-soluble. In this report, the production of exochelin in M. neoaurum was induced in iron-deficiency, but repressed under iron-sufficiency growth conditions. It is however not induced under zinc-deficiency growth conditions. The growth of this mycobacterium was correlated with exochelin secretion under iron-deficiency culture conditions. When M. neoaurum was grown in defined medium containing 0.04 μg Fe(III)/mL (final concentration), the production of exochelin reached a maximum and the corresponding cell growth was comparable to that under iron-sufficiency conditions. In this study, exochelin was purified from spent supernatant of M. neoaurum bysemi-preparative chromatography. When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed. It is proposed that iron uptake in M. neoaurum is exochelin-mediated.
Keywords: Mycobacterium neoaurum; siderophore; exochelin; iron-deficiency; ferri-exochelin; zinc-deficiency
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MDPI and ACS Style
Chan, K.-G. Exochelin Production in Mycobacterium neoaurum. Int. J. Mol. Sci. 2009, 10, 345-353.
Chan K-G. Exochelin Production in Mycobacterium neoaurum. International Journal of Molecular Sciences. 2009; 10(1):345-353.
Chan, Kok-Gan. 2009. "Exochelin Production in Mycobacterium neoaurum." Int. J. Mol. Sci. 10, no. 1: 345-353.