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Int. J. Mol. Sci. 2009, 10(1), 345-353; doi:10.3390/ijms10010345

Exochelin Production in Mycobacterium neoaurum

1
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
2
Centre for Research in Biotechnology for Agriculture (CEBAR), Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia
Received: 26 September 2008 / Revised: 13 January 2009 / Accepted: 15 January 2009 / Published: 20 January 2009
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37ºC within 3 – 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most mycobacteria produce siderophores. One example is exochelin, which is extracellular and water-soluble. In this report, the production of exochelin in M. neoaurum was induced in iron-deficiency, but repressed under iron-sufficiency growth conditions. It is however not induced under zinc-deficiency growth conditions. The growth of this mycobacterium was correlated with exochelin secretion under iron-deficiency culture conditions. When M. neoaurum was grown in defined medium containing 0.04 μg Fe(III)/mL (final concentration), the production of exochelin reached a maximum and the corresponding cell growth was comparable to that under iron-sufficiency conditions. In this study, exochelin was purified from spent supernatant of M. neoaurum bysemi-preparative chromatography. When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed. It is proposed that iron uptake in M. neoaurum is exochelin-mediated.
Keywords: Mycobacterium neoaurum; siderophore; exochelin; iron-deficiency; ferri-exochelin; zinc-deficiency Mycobacterium neoaurum; siderophore; exochelin; iron-deficiency; ferri-exochelin; zinc-deficiency
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Chan, K.-G. Exochelin Production in Mycobacterium neoaurum. Int. J. Mol. Sci. 2009, 10, 345-353.

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