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Exochelin Production in Mycobacterium neoaurum
AbstractMycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37ºC within 3 – 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most mycobacteria produce siderophores. One example is exochelin, which is extracellular and water-soluble. In this report, the production of exochelin in M. neoaurum was induced in iron-deficiency, but repressed under iron-sufficiency growth conditions. It is however not induced under zinc-deficiency growth conditions. The growth of this mycobacterium was correlated with exochelin secretion under iron-deficiency culture conditions. When M. neoaurum was grown in defined medium containing 0.04 μg Fe(III)/mL (final concentration), the production of exochelin reached a maximum and the corresponding cell growth was comparable to that under iron-sufficiency conditions. In this study, exochelin was purified from spent supernatant of M. neoaurum bysemi-preparative chromatography. When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed. It is proposed that iron uptake in M. neoaurum is exochelin-mediated.
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Chan, K.-G. Exochelin Production in Mycobacterium neoaurum. Int. J. Mol. Sci. 2009, 10, 345-353.View more citation formats
Chan K-G. Exochelin Production in Mycobacterium neoaurum. International Journal of Molecular Sciences. 2009; 10(1):345-353.Chicago/Turabian Style
Chan, Kok-Gan. 2009. "Exochelin Production in Mycobacterium neoaurum." Int. J. Mol. Sci. 10, no. 1: 345-353.