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Molecules 2003, 8(1), 120-126; doi:10.3390/80100120
Article

A New Potent Route of DNA Vaccine Inoculation: DNA-Liposome Complexes on Bare Skin Induce Antigen-Special Antibody Responses

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, , ,  and *
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, P.R.China
* Author to whom correspondence should be addressed.
Received: 1 January 2003 / Published: 31 January 2003
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Abstract

Transcutaneous immunization is a novel strategy for genetic vaccine immunization to induce detectable antigen-special antibody in humor and mucosal. In this study, plasmid expressing hepatitis B surface antigen (pGFP-HBsAg) was encapsulated in liposome, then DNA- liposome complexes were glued on bare skin of mice ear in different dosage (50μg, 10μg and 1μg). As control, DNA- liposome complexes of pGFP-HBsAg and pGFP vector were inoculated intraperitoneally. The anti-HBsAg antibodies of serum were detected weekly by ELISA. It was found that the detectable antibodies of transcutaneous immunized mouse were elicited after four weeks, and reached a maximum at the sixth week. Even 1μg plasmid DNA in liposomes through immune skin can elicit the highest ELISA antibody titer (> 1:512) in test group, and corresponding percentage of positive response is up to 71% at sixth week, but higher amounts of plasmid DNA (50μg DNA per mice) on immune skin cannot induce higher antibody levels. The result showed that DNA- liposome complexes glued on bare skin appear to be a novel method for the administration of DNA vaccines.
Keywords: genetic vaccine; liposome; transcutaneous immunization genetic vaccine; liposome; transcutaneous immunization
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Liang, R.; Zhuang, F.; Meng, Z.; Deng, M.; Zheng, C.; Duan, M. A New Potent Route of DNA Vaccine Inoculation: DNA-Liposome Complexes on Bare Skin Induce Antigen-Special Antibody Responses. Molecules 2003, 8, 120-126.

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