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• Wroczynski, P
• Wierzchowski, J
• Golab, J
• Blazejczyk, M
• Borecka, D
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• Wroczynski, P
• Wierzchowski, J
• Golab, J
• Blazejczyk, M
• Borecka, D
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• Wroczynski, P
• Wierzchowski, J
• Golab, J
• Blazejczyk, M
• Borecka, D

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Molecules 2002, 7(12), 896-901; doi:10.3390/71200896

Article

Determination of Aldehyde Dehydrogenase (ALDH) Isozymes in Human Cancer Samples - Comparison of Kinetic and Immunochemical Assays

Piotr Wroczynski ^1,* , Jacek Wierzchowski ² , Jakub Golab ³ , Magdalena Blazejczyk ¹  and Dorota Borecka ¹

¹ Laboratory of Organic Chemistry, Faculty of Pharmacy and Department of Immunology, Medical University, 1 Banacha St., Warsaw, PL-02097, Poland ² Institute of Chemistry, University of Podlasie, 54 Trzeciego Maja St., Siedlce, PL-08-110, Poland ³ Department of Immunology, Medical University, 2 Oczki St., Warsaw, PL-00-052, Poland

* Author to whom correspondence should be addressed.

Received: 25 October 2002 / Accepted: 4 December 2002 / Published: 31 December 2002

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Abstract: A fluorimetric assay of aldehyde dehydrogenase isozymes, based on naphthaldehyde oxidation, is compared with Western Blotting analysis on several clinical samples obtained from surgery. The comparison reveals qualitatively good correlation of ALDH1A1 isozyme detection with two methods and somewhat worse on ALDH3A1 assay.

Keywords: Aldehyde dehydrogenase; isozymes; western blotting; fluorimetric assays

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