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Molecules 2018, 23(9), 2358; https://doi.org/10.3390/molecules23092358

Ultra-Small Pd(0) Nanoparticles into a Designed Semisynthetic Lipase: An Efficient and Recyclable Heterogeneous Biohybrid Catalyst for the Heck Reaction under Mild Conditions

1
Department of Biocatalysis, Institute of Catalysis (CSIC), Marie Curie 2, Cantoblanco Campus UAM, 28049 Madrid, Spain
2
Institute of Food Science, Technology and Nutrition (ICTAN-CSIC), José Antonio Novais 10, 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Received: 27 July 2018 / Revised: 13 September 2018 / Accepted: 13 September 2018 / Published: 14 September 2018
(This article belongs to the Special Issue Biomolecular Catalysts)
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Abstract

A novel heterogeneous enzyme-palladium (Pd) (0) nanoparticles (PdNPs) bionanohybrid has been synthesized by an efficient, green, and straightforward methodology. A designed Geobacillus thermocatenulatus lipase (GTL) variant genetically and then chemically modified by the introduction of a tailor-made cysteine-containing complementary peptide- was used as the stabilizing and reducing agent for the in situ formation of ultra-small PdNPs nanoparticles embedded on the protein structure. This bionanohybrid was an excellent catalyst in the synthesis of trans-ethyl cinnamate by Heck reaction at 65 °C. It showed the best catalytic performance in dimethylformamide (DMF) containing 10–25% of water as a solvent but was also able to catalyze the reaction in pure DMF or with a higher amount of water as co-solvent. The recyclability and stability were excellent, maintaining more than 90% of catalytic activity after five cycles of use. View Full-Text
Keywords: heterogeneous catalyst; palladium nanoparticles; semisynthetic enzyme; lipase; Heck reaction heterogeneous catalyst; palladium nanoparticles; semisynthetic enzyme; lipase; Heck reaction
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Lopez-Tejedor, D.; de las Rivas, B.; Palomo, J.M. Ultra-Small Pd(0) Nanoparticles into a Designed Semisynthetic Lipase: An Efficient and Recyclable Heterogeneous Biohybrid Catalyst for the Heck Reaction under Mild Conditions. Molecules 2018, 23, 2358.

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