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Molecules 2018, 23(9), 2128; https://doi.org/10.3390/molecules23092128

In Vitro Ecological Response of the Human Gut Microbiome to Bioactive Extracts from Edible Wild Mushrooms

1
University of Agronomic Science and Veterinary Medicine, Faculty of Biotechnology, 59 Marasti Blvd, 1 District, 011464 Bucharest, Romania
2
Centre of Bioanalysis, National Institute for Biological Sciences, 296 Spl. Independentei, 060031 Bucharest, Romania
3
ICUB-Research Institute of the University of Bucharest, 36–46 Bd. M. Kogalniceanu, 5th District, 050107 Bucharest, Romania
*
Author to whom correspondence should be addressed.
Received: 11 July 2018 / Revised: 17 August 2018 / Accepted: 22 August 2018 / Published: 23 August 2018
(This article belongs to the Special Issue Bioactive Plant Compounds for Sustainable Health)
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Abstract

This study presents the effect of two new products based on atomized extracts from edible wild mushrooms (RoBioMush1, RoBioMush2) on the microbiota of three target groups: clinically healthy (NG) individuals, individuals with nutritional disorders (ND), and individuals with cardiovascular diseases (CVD). The microbiota fingerprints were determined by quantitative polymerase chain reaction (qPCR). Modulations in the simulated microbiome were established and correlated with the presence of phenolic compounds released in the in vitro environment (a three-stage culture system GIS2 simulator, www.gissystems.ro). The high metabolizing capacity of NG and CVD correlated positively with the rest of the biological activities expressed in vitro. ND microbiota consumed a wide spectrum of monosaccharides from the products. Xylose was present in large quantities in the descending segment (minimum: 175 μg/mL for ND). The primary conclusion was that the microbiological ecosystem was modulated, as proven by the presence of specific biomarkers (e.g., ammonium levels and fingerprints of short-chain fatty acids–SCFAs), which stimulate the organism’s health status and were correlated with the restoration of a normal microbiota fingerprint. View Full-Text
Keywords: gallic acid; mushroom; microbiota; Boletus; in vitro; three stage culture system gallic acid; mushroom; microbiota; Boletus; in vitro; three stage culture system
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Vamanu, E.; Gatea, F.; Sârbu, I. In Vitro Ecological Response of the Human Gut Microbiome to Bioactive Extracts from Edible Wild Mushrooms. Molecules 2018, 23, 2128.

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