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Molecules 2018, 23(8), 2049; https://doi.org/10.3390/molecules23082049

An In Vitro Estimation of the Cytotoxicity and Genotoxicity of Root Extract from Leonurus sibiricus L. Overexpressing AtPAP1 against Different Cancer Cell Lines

1
Department of Biology and Pharmaceutical Botany, Medical University of Łódź, Muszyńskiego 1, 90-151 Łódź, Poland
2
Laboratory of Medical Genetics, Faculty of Biology and Environmental Protection, University of Łódź, Pomorska 141/143, 90-236 Łódź, Poland
3
Department of Genetics and Plant Molecular Biology and Biotechnology, The University of Łódź, Banacha 12/13, 90-237 Łódź, Poland
*
Author to whom correspondence should be addressed.
Received: 20 July 2018 / Revised: 8 August 2018 / Accepted: 12 August 2018 / Published: 16 August 2018
(This article belongs to the Collection Herbal Medicine Research)
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Abstract

As the current cancer treatment success rate is not sufficient, interest has grown in plants as possible sources of anti-cancer compounds. One such plant with a broad spectrum of activity is Lenourus sibiricus of the family Lamiaceae. This study investigates for the first time both the genotoxic and cytotoxic activities of TR (transformed) and AtPAP1 TR (with over-expression of transcriptional factor) root extracts of Lenourus sibiricus against various cancer cell lines (CCRF-CEM, K-562 and A549). Both tested extracts showed a cytotoxic effect on CCRF-CEM and K-562 cell lines, but strongest activity was observed for the AtPAP1 TR extract. No cytotoxic effect was observed against the A549 cell line in the tested concentration range, and it was found that both tested extracts may induce apoptosis by decreasing mitochondrial membrane potential and inducing nDNA damage lesion in the TP53 region and mtDNA in ND1 (mitochondrially encoded NADH: ubiquinone oxidoreductase core subunit 1) and ND5 (mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 5) regions in K-562 and CCRF-CEM. Our results confirmed that TR and AtPAP1 TR root extracts from L. sibiricus are cytotoxic and genotoxic against different model cell lines (CCRF-CEM and K-562). However, the observed genotoxicity of both extracts needs to be confirmed by additional studies. These preclinical observations support the use of L. sibiricus with other pharmacological purposes. View Full-Text
Keywords: AtPAP1 transgenic roots of Leonurus sibiricus; cytotoxic effect; mtDNA copy number; mitochondrial membrane potential; genotoxic effect AtPAP1 transgenic roots of Leonurus sibiricus; cytotoxic effect; mtDNA copy number; mitochondrial membrane potential; genotoxic effect
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Sitarek, P.; Synowiec, E.; Kowalczyk, T.; Śliwiński, T.; Skała, E. An In Vitro Estimation of the Cytotoxicity and Genotoxicity of Root Extract from Leonurus sibiricus L. Overexpressing AtPAP1 against Different Cancer Cell Lines. Molecules 2018, 23, 2049.

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