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Molecules 2018, 23(8), 1984; https://doi.org/10.3390/molecules23081984

Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic Weissella cibaria, CMU

1
Department of Dental Education, School of Dentistry, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Korea
2
Research Institute, Oradentics Inc., 1805-ho, 25 Seongsuil-ro-4-gil, Seongdong-gu, Seoul 04781, Korea
3
Division of Strategic Food Research, Korea Food Research Institute (KFRI), 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, Korea
*
Author to whom correspondence should be addressed.
Received: 11 July 2018 / Revised: 4 August 2018 / Accepted: 5 August 2018 / Published: 9 August 2018
(This article belongs to the Special Issue Antibacterial Materials and Coatings 2018)
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Abstract

Recently, studies have explored the use of probiotics like the Weissella cibaria strain, CMU (oraCMU), for use as preventive dental medicine instead of chemical oral care methods. The present study was conducted to investigate the antibacterial properties of the cell-free supernatant (CFS) from this bacterium. Cell morphology using the scanning electron microscope, and the antibacterial effect of CFS under various growth conditions were evaluated. The production of hydrogen peroxide, organic acids, fatty acids, and secretory proteins was also studied. Most of the antibacterial effects of oraCMU against periodontal pathogens were found to be acid- and hydrogen peroxide-dose-dependent effects. Lactic acid, acetic acid, and citric acid were the most common organic acids. Among the 37 fatty acids, only 0.02% of oleic acid (C18:1n-9, cis) was detected. Proteomic analysis of the oraCMU secretome identified a total of 19 secreted proteins, including N-acetylmuramidase. This protein may be a potential anti-microbial agent effective against Porphyromonas gingivalis. View Full-Text
Keywords: Weissella cibaria; antibacterial; cell-free supernatant; organic acid; secretome; probiotic Weissella cibaria; antibacterial; cell-free supernatant; organic acid; secretome; probiotic
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Lim, H.-S.; Yeu, J.-E.; Hong, S.-P.; Kang, M.-S. Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic Weissella cibaria, CMU. Molecules 2018, 23, 1984.

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