Next Article in Journal
Synthesis of New 1,3,5-Triazine-Based 2-Pyrazolines as Potential Anticancer Agents
Previous Article in Journal
Synthesis of Novel 1,4-Naphthoquinones Possessing Indole Scaffolds Using In(OTf)3 in Solvent-Free Conditions
Article Menu

Export Article

Open AccessArticle
Molecules 2018, 23(8), 1955; https://doi.org/10.3390/molecules23081955

Internalization of Titanium Dioxide Nanoparticles Is Cytotoxic for H9c2 Rat Cardiomyoblasts

1
Departamento de Fisiología (Biología Celular), Instituto Nacional de Cardiología “Ignacio Chávez”, Juan Badiano No. 1, Colonia Sección XVI, Tlalpan, C.P. 14080, Ciudad de México, Mexico
2
Swetox, Karolinska Institutet, Unit of Toxicology Sciences, Forskargatan 20, SE-151 36 Södertälje, Sweden
3
Departamento de Microscopía Electrónica, Instituto Nacional de Cancerología, Av. San Fernando No. 22, Colonia Sección XVI, Tlalpan, C.P. 14080 Ciudad de México, Mexico
*
Author to whom correspondence should be addressed.
Received: 29 June 2018 / Revised: 24 July 2018 / Accepted: 1 August 2018 / Published: 6 August 2018
(This article belongs to the Section Nanochemistry)
View Full-Text   |   Download PDF [4118 KB, uploaded 6 August 2018]   |  

Abstract

Titanium dioxide nanoparticles (TiO2 NPs) are widely used in industry and daily life. TiO2 NPs can penetrate into the body, translocate from the lungs into the circulation and come into contact with cardiac cells. In this work, we evaluated the toxicity of TiO2 NPs on H9c2 rat cardiomyoblasts. Internalization of TiO2 NPs and their effect on cell proliferation, viability, oxidative stress and cell death were assessed, as well as cell cycle alterations. Cellular uptake of TiO2 NPs reduced metabolic activity and cell proliferation and increased oxidative stress by 19-fold measured as H2DCFDA oxidation. TiO2 NPs disrupted the plasmatic membrane integrity and decreased the mitochondrial membrane potential. These cytotoxic effects were related with changes in the distribution of cell cycle phases resulting in necrotic death and autophagy. These findings suggest that TiO2 NPs exposure represents a potential health risk, particularly in the development of cardiovascular diseases via oxidative stress and cell death. View Full-Text
Keywords: titanium dioxide nanoparticles; cardiomyoblasts; internalization; oxidative stress; necrosis; autophagy titanium dioxide nanoparticles; cardiomyoblasts; internalization; oxidative stress; necrosis; autophagy
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Huerta-García, E.; Zepeda-Quiroz, I.; Sánchez-Barrera, H.; Colín-Val, Z.; Alfaro-Moreno, E.; Ramos-Godinez, M.P.; López-Marure, R. Internalization of Titanium Dioxide Nanoparticles Is Cytotoxic for H9c2 Rat Cardiomyoblasts. Molecules 2018, 23, 1955.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top