Next Article in Journal
Quantitative Determination of Thiabendazole in Soil Extracts by Surface-Enhanced Raman Spectroscopy
Previous Article in Journal
AIE/ACQ Effects in Two DR/NIR Emitters: A Structural and DFT Comparative Analysis
Article Menu

Export Article

Open AccessArticle
Molecules 2018, 23(8), 1948; https://doi.org/10.3390/molecules23081948

Structure Characterization and Hypoglycaemic Activities of Two Polysaccharides from Inonotus obliquus

1
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
2
Bio-Pharmaceutical College, Beijing City University, Beijing 100094, China
3
College of Life Sciences, Qufu Normal University, Jining 273165, China
*
Author to whom correspondence should be addressed.
Academic Editor: Tommasina Coviello
Received: 29 June 2018 / Revised: 29 July 2018 / Accepted: 2 August 2018 / Published: 4 August 2018
View Full-Text   |   Download PDF [2652 KB, uploaded 4 August 2018]   |  

Abstract

In the present study, two polysaccharides (HIOP1-S and HIOP2-S) were isolated and purified from Inonotus obliquus using DEAE-52 cellulose and Sephadex G-100 column chromatography. The structural characterization and in vitro and in vivo hypoglycaemic activities of these molecules were investigated. HPLC analysis HIOP1-S was a heterpolysaccharide with glucose and galactose as the main compontent monosaccharides (50.247%, molar percentages). However, HIOP2-S was a heterpolysaccharide with glucose as the main monosaccharide (49.881%, molar percentages). The average molecular weights of HIOP1-S and HIOP2-S were 13.6 KDa and 15.2 KDa, respectively. The β-type glycosidic bond in HIOP1-S and HIOP2-S was determined using infrared analysis. 1H-NMR spectra indicated that HIOP2-S contains the β-configuration glycosidic bond, and the glycoside bonds of HIOP1-S are both α-type and β-type. The ultraviolet scanning showed that both HIOP1-S and HIOP2-S contained a certain amount of binding protein. Congo red test showed that HIOP1-S and HIOP2-S could form a regular ordered triple helix structure in the neutral and weakly alkaline range. HIOP1-S and HIOP2-S showed strong α-glucosidase inhibitory activities and increased the glucose consumption of HepG2 cells. In addition, Streptozotocin (STZ)-induced hyperglycaemic mice were used to evaluate the antihyperglycaemic effects of HIOP1-S and HIOP2-S in vivo. The results showed that HIOP2-S had antihyperglycaemic effects. Taken together, these results suggest that HIOP1-S and HIOP2-S have potential anti-diabetic effects. View Full-Text
Keywords: Inonotus obliquus; polysaccharide; structure; α-glucosidase inhibitory activity; HepG2 cells Inonotus obliquus; polysaccharide; structure; α-glucosidase inhibitory activity; HepG2 cells
Figures

Figure 1a

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Liu, P.; Xue, J.; Tong, S.; Dong, W.; Wu, P. Structure Characterization and Hypoglycaemic Activities of Two Polysaccharides from Inonotus obliquus. Molecules 2018, 23, 1948.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top