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Molecules 2018, 23(8), 1912; https://doi.org/10.3390/molecules23081912

Physicochemical Characterization, Antioxidant and Immunostimulatory Activities of Sulfated Polysaccharides Extracted from Ascophyllum nodosum

1,2,3
,
1
,
2
,
1,2,3
,
2,3,* , 4
and
1,*
1
College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China
2
School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China
3
Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland, Yancheng Institute of Technology, Yancheng 224051, China
4
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
*
Authors to whom correspondence should be addressed.
Received: 5 July 2018 / Revised: 24 July 2018 / Accepted: 26 July 2018 / Published: 31 July 2018
(This article belongs to the Collection Bioactive Compounds)
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Abstract

Polysaccharides from Ascophyllum nodosum (AnPS) were extracted and purified via an optimized protocol. The optimal extraction conditions were as follows: extraction time of 4.3 h, extraction temperature of 84 °C and ratio (v/w, mL/g) of extraction solvent (water) to raw material of 27. The resulting yield was 9.15 ± 0.23% of crude AnPS. Two fractions, named AnP1-1 and AnP2-1 with molecular weights of 165.92 KDa and 370.68 KDa, were separated from the crude AnPS by chromatography in DEAE Sepharose Fast Flow and Sephacryl S-300, respectively. AnP1-1 was composed of mannose, ribose, glucuronic acid, glucose and fucose, and AnP2-1 was composed of mannose, glucuronic acid, galactose and fucose. AnPS, AnP1-1 and AnP2-1 exhibited high scavenging activities against ABTS radical and superoxide radical, and showed protective effect on H2O2-induced oxidative injury in RAW264.7 cells. Furthermore, the immunostimulatory activities of AnP1-1 and AnP2-1 were evaluated by Caco-2 cells, the results showed both AnP1-1 and AnP2-1 could significantly promote the production of immune reactive molecules such as interleukin (IL)-8, IL-1β, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α. Therefore, the results suggest that AnPS and its two fractions may be explored as a potential functional food supplement. View Full-Text
Keywords: Ascophyllum nodosum; polysaccharides; antioxidant activity; immunostimulatory activity Ascophyllum nodosum; polysaccharides; antioxidant activity; immunostimulatory activity
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Chen, L.; Wang, Y.; Yang, H.; Li, H.; Xu, W.; Chen, G.; Zhu, H. Physicochemical Characterization, Antioxidant and Immunostimulatory Activities of Sulfated Polysaccharides Extracted from Ascophyllum nodosum. Molecules 2018, 23, 1912.

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