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Molecules 2018, 23(5), 1144; https://doi.org/10.3390/molecules23051144

The Sucrose Synthase Gene Family in Chinese Pear (Pyrus bretschneideri Rehd.): Structure, Expression, and Evolution

1
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China
2
School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
*
Author to whom correspondence should be addressed.
Received: 20 March 2018 / Revised: 27 April 2018 / Accepted: 6 May 2018 / Published: 11 May 2018
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Abstract

Sucrose synthase (SS) is a key enzyme involved in sucrose metabolism that is critical in plant growth and development, and particularly quality of the fruit. Sucrose synthase gene families have been identified and characterized in plants various plants such as tobacco, grape, rice, and Arabidopsis. However, there is still lack of detailed information about sucrose synthase gene in pear. In the present study, we performed a systematic analysis of the pear (Pyrus bretschneideri Rehd.) genome and reported 30 sucrose synthase genes. Subsequently, gene structure, phylogenetic relationship, chromosomal localization, gene duplications, promoter regions, collinearity, RNA-Seq data and qRT-PCR were conducted on these sucrose synthase genes. The transcript analysis revealed that 10 PbSSs genes (30%) were especially expressed in pear fruit development. Additionally, qRT-PCR analysis verified the RNA-seq data and shown that PbSS30, PbSS24, and PbSS15 have a potential role in the pear fruit development stages. This study provides important insights into the evolution of sucrose synthase gene family in pear and will provide assistance for further investigation of sucrose synthase genes functions in the process of fruit development, fruit quality and resistance to environmental stresses. View Full-Text
Keywords: sucrose synthase; characteristics; phylogenetic analysis; RNA-seq data analysis; expression pattern sucrose synthase; characteristics; phylogenetic analysis; RNA-seq data analysis; expression pattern
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Abdullah, M.; Cao, Y.; Cheng, X.; Meng, D.; Chen, Y.; Shakoor, A.; Gao, J.; Cai, Y. The Sucrose Synthase Gene Family in Chinese Pear (Pyrus bretschneideri Rehd.): Structure, Expression, and Evolution. Molecules 2018, 23, 1144.

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