Next Article in Journal
Investigation of the Antifungal Activity and Mode of Action of Thymus vulgaris, Citrus limonum, Pelargonium graveolens, Cinnamomum cassia, Ocimum basilicum, and Eugenia caryophyllus Essential Oils
Previous Article in Journal
Identifying Cancer Specific Driver Modules Using a Network-Based Method
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Molecules 2018, 23(5), 1115; https://doi.org/10.3390/molecules23051115

Tissue-Specific Analysis of Secondary Metabolites Creates a Reliable Morphological Criterion for Quality Grading of Polygoni Multiflori Radix

School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China
*
Author to whom correspondence should be addressed.
Received: 9 April 2018 / Revised: 28 April 2018 / Accepted: 6 May 2018 / Published: 8 May 2018
View Full-Text   |   Download PDF [10306 KB, uploaded 8 May 2018]   |  

Abstract

In commercial herbal markets, Polygoni Multiflori Radix (PMR, the tuberous roots of Polygonum multiflorum Thunb.), a commonly-used Chinese medicinal material, is divided into different grades based on morphological features of size and weight. While more weight and larger size command a higher price, there is no scientific data confirming that the more expensive roots are in fact of better quality. To assess the inherent quality of various grades and of various tissues in PMR and to find reliable morphological indicators of quality, a method combining laser microdissection (LMD) and ultra-performance liquid chromatography triple-quadrupole mass spectrometry (UPLC-QqQ-MS/MS) was applied. Twelve major chemical components were quantitatively determined in both whole material and different tissues of PMR. Determination of the whole material revealed that traditional commercial grades based on size and weight of PRM did not correspond to any significant differences in chemical content. Instead, tissue-specific analysis indicated that the morphological features could be linked with quality in a new way. That is, PMR with broader cork and phloem, as seen in a transverse section, were typically of better quality as these parts are where the bioactive components accumulate. The tissue-specific analysis of secondary metabolites creates a reliable morphological criterion for quality grading of PMR. View Full-Text
Keywords: Polygoni Multiflori Radix; laser microdissection; ultra-performance liquid chromatography triple-quadrupole mass spectrometry (UPLC-QqQ-MS/MS); secondary metabolites; quality grading Polygoni Multiflori Radix; laser microdissection; ultra-performance liquid chromatography triple-quadrupole mass spectrometry (UPLC-QqQ-MS/MS); secondary metabolites; quality grading
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Share & Cite This Article

MDPI and ACS Style

Liang, L.; Xu, J.; Liang, Z.-T.; Dong, X.-P.; Chen, H.-B.; Zhao, Z.-Z. Tissue-Specific Analysis of Secondary Metabolites Creates a Reliable Morphological Criterion for Quality Grading of Polygoni Multiflori Radix. Molecules 2018, 23, 1115.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top