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Molecules 2018, 23(5), 1109; https://doi.org/10.3390/molecules23051109

Efficient Approach for the Extraction and Identification of Red Pigment from Zanthoxylum bungeanum Maxim and Its Antioxidant Activity

1,†
,
1,†
,
1
,
1
,
1
,
1
and
1,2,*
1
College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
2
Key Research Laboratory of Traditional Chinese Medicine Processing Technology, State Administration of Traditional Chinese Medicine of China, Chengdu 611137, China
These authors contributed equally to this manuscript.
*
Author to whom correspondence should be addressed.
Academic Editor: Tsukasa Iwashina
Received: 10 April 2018 / Revised: 25 April 2018 / Accepted: 27 April 2018 / Published: 8 May 2018
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Abstract

Red pigment (RP) was extracted from the peels of Zanthoxylum bungeanum Maxim (PZB) by ultrasonic-assisted extraction (UAE) in this work. Box–Behnken design–response surface methodology (BBD-RSM) was employed to research the efficiency of the RP extraction. Based on the optimization of RSM, results showed that the optimal extraction conditions were as follows: liquid–solid ratio of 31 mL/g, extraction time of 41 min, and extraction temperature of 27 °C, and under these conditions, the actual absorbance value was 0.615 ± 0.13%, highly agreeing with the predicted value by the model. Furthermore, ultra-performance liquid chromatography–mass spectrometry (UPLC-MS) was used to separate and analyze RP. The compounds of RP were mainly flavonoids, and there were five compounds detected for the first time in PZB. In addition, RP showed significant antioxidant activities in vitro, which could be developed for anti-aging candidate drugs and functional foods. In conclusion, ultrasound-assisted extraction with BBD-RSM and chromatographic separation technology with UPLC-MS are efficient strategies for the isolation and identification of RP from PZB. View Full-Text
Keywords: Zanthoxylum bungeanum Maxim; red pigment; ultrasound-assisted extraction; BBD-RSM; UPLC-MS; antioxidant activity Zanthoxylum bungeanum Maxim; red pigment; ultrasound-assisted extraction; BBD-RSM; UPLC-MS; antioxidant activity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Chen, X.; Wei, Z.; Zhu, L.; Yuan, X.; Wei, D.; Peng, W.; Wu, C. Efficient Approach for the Extraction and Identification of Red Pigment from Zanthoxylum bungeanum Maxim and Its Antioxidant Activity. Molecules 2018, 23, 1109.

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