Next Article in Journal
Identification of Ophiocordyceps sinensis and Its Artificially Cultured Ophiocordyceps Mycelia by Ultra-Performance Liquid Chromatography/Orbitrap Fusion Mass Spectrometry and Chemometrics
Previous Article in Journal
Effects of 200 Gy 60Co-γ Radiation on the Regulation of Antioxidant Enzymes, Hsp70 Genes, and Serum Molecules of Plutella xylostella (Linnaeus)
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 23
Issue 5
10.3390/molecules23051012
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Determination of Arsenic Species in Ophiocordyceps sinensis from Major Habitats in China by HPLC-ICP-MS and the Edible Hazard Assessment

by
Lian-Xian Guo
1,†,
Gui-Wei Zhang
2,†,
Jia-Ting Wang
1,
Yue-Ping Zhong
1 and
Zhi-Gang Huang
1,*
1
Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, China
2
Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518000, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2018, 23(5), 1012; https://doi.org/10.3390/molecules23051012
Submission received: 2 April 2018 / Revised: 21 April 2018 / Accepted: 24 April 2018 / Published: 26 April 2018
(This article belongs to the Section Analytical Chemistry)
Browse Figures
Versions Notes

Abstract

:
This study sought to determine the concentration and distribution of arsenic (As) species in Ophiocordyceps sinensis (O. sinensis), and to assess its edible hazard for long term consumption. The total arsenic concentrations, measured through inductively coupled plasma mass spectrometry (ICP-MS), ranged from 4.00 mg/kg to 5.25 mg/kg. As determined by HPLC-ICP-MS, the most concerning arsenic species—AsB, MMAV, DMAV, AsV, and AsШ—were either not detected (MMAV and DMAV) or were detected as minor As species (AsB: 1.4–2.9%; AsV: 1.3–3.2%, and AsШ: 4.1–6.0%). The major components were a cluster of unknown organic As (uAs) compounds with AsШ, which accounted for 91.7–94.0% of the As content. Based on the H2O2 test and the chromatography behavior, it can be inferred that, the uAs might not be toxic organic As. Estimated daily intake (EDI), hazard quotient (HQ), and cancer risk (CR) caused by the total As content; the sum of inorganic As (iAs) and uAs, namely i+uAs; and iAs exposure from long term O. sinensis consumption were calculated and evaluated through equations from the US Environmental Protection Agency and the uncertainties were analyzed by Monte-Carlo Simulation (MCS). EDItotal As and EDIi+uAs are approximately ten times more than EDIiAs; HQtotal As and HQi+uAs > 1 while HQiAs < 1; and CRtotal As and CRi+uAs > 1 × 10−4 while CRiAs < 1 × 10−4. Thus, if the uAs is non-toxic, there is no particular risk to local consumers and the carcinogenic risk is acceptable for consumption of O. sinensis because the concentration of toxic iAs is very low.

1. Introduction

Ophiocordyceps sinensis (O. sinensis), a mysterious entomogenous fungus distributed on the Qinghai–Tibet Plateau, is popularly referred to as winter-worm-summer-grass (Dong Chong Xia Cao in Chinese) [1,2] (Figure 1). O. sinensis has been utilized in China (Figure 2A) and surrounding countries for more than 2000 years as a rare functional food to promote health and to treat diverse chronic diseases. In 1993, O. sinensis became famous worldwide because Chinese female athletes broke several world records in running events at the National Games and the meritorious performances were later attributed (at least in part) to the consumption of this fungus [3]. Subsequently, modern pharmaceutical research has shown that its predominant functions are anti-tumor, anti-inflammatory, nephroprotective, antioxidant, antihyperglycemic, anti-apoptosis, immunoregulatory, and hepatoprotective [4,5]. Accordingly, these pronounced medicinal functions have resulted in a large demand for wild O. sinensis and have also increased the local prosperity of economically poor production areas around the Qinghai–Tibet Plateau and in adjacent countries. Some households earn as much as two-thirds of their income from the collection of O. sinensis (Figure 2B), and pastoralists are earning an income of a size unrecorded in their history [6,7]. However, concerns about human health have arisen since the CFDA (China Food and Drug Administration) revealed in 2016 that excessive Arsenic (As) content (total As: 4.4–9.0 mg/kg [8]) was detected in O. sinensis, a level five times the reference value of 1 mg/kg for total As in functional foods (GB16740-2014) [9]. Subsequently, the CFDA ordered that all the pilot work on O. sinensis as a functional food be discontinued on 26 February 2016 [10]. Moreover, numerous media outlets had arbitrarily declared that O. sinensis is a poison instead of a functional food. This assertion has caused a great uproar in the health food market and has seriously affected the O. sinensis-dependent economic chain [11].
Arsenic can be present in both organic and inorganic forms. The toxicity of As is dependent on its chemical form, with inorganic species (iAs), such as arsenite (AsШ) and arsenate (AsV), being the most toxic [12]. Organic species are the metabolic products of iAs, such as monomethylarsonic acid (MMAV) and dimethylarsenic acid (DMAV), and are much less toxic than iAs to humans. Additionally, some other organic As complexes (arsenocholine, arsenobetaine, various arsenosugars and arsenolipids) are generally considered nontoxic [13], according to previous studies on As toxicity. However, recently, experimental results have documented the presence of trivalent intermediates, monomethylarsonous acid (MMAШ) and dimethylarsinous acid (DMAШ) in the urine of humans exposed to drinking water containing high levels of inorganic As [14]. These trivalent intermediates are structurally different from the pentavalent compounds and are more reactive and more carcinogenic [15,16,17]. More recently, the subsequent metabolic products of DMAШ, sulfur-containing intermediary metabolites (dimethylmonothioarsinicacid, DMMTAV) [18], and several As containing hydrocarbons (AsHC 332, AsHC 360 and AsHC 444) were shown to high toxic effects on organisms [19]. The different toxicities of As species reinforce the importance of distinguishing its chemical form, as the total amount of As does not provide enough information about the toxicity of the analyzed sample. Therefore, it is incorrect to consider O. sinensis is toxic according to its total As content. Unlike previous reported arsenic accumulated mushrooms, which germinated on plant sourced media, O. sinensis only lives in Thitarodes larva of 4–5th instar. Inspired by the significantly higher organic As proportion in animal-sourced traditional Chinese medicine (TCM) compared to plant-sourced TCM [20], some scholars have proposed that due to larva-fungus complexity (Figure 2C,D), O. sinensis might contain a large proportion of organic As. Recently, researchers have provided evidence for the speculation that iAs species were minor (AsШ and AsV) or below the level of detection after examining the As speciation in O. sinensis through high-performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS). In addition, they found that the largest proportion of As was composed of an unknown organic As (uAs) species [21]. Because of the shortcomings of conventional HPLC-HG-AFS, in that research, they only discriminated four As species (MMAV, DMAV, AsV and AsШ) in O. sinensis samples [21]. Non-forming hydride species, such as AsB, important in biota samples, cannot be evaluated using this approach [22].
To further clarify the As speciation in O. sinensis and oral intake hazards in longtime consumption, the present study examined the total As content, including the most concerning As species (AsШ, AsV MMAV, DMAV, and AsB), in O. sinensis using the most commonly applied techniques: inductively coupled plasma mass spectrometry (ICP-MS) and anion exchange high-performance liquid chromatography–inductively coupled plasma mass spectrometry (HPLC-ICP-MS) [23,24]. Due to the extraordinary prices of the O. sinensis and chronic injury effects to organisms due to Arsenic consumption, a hazard assessment via experimental animal trials would be costly. Thus, in this study, we chose the most widely accepted model (Environment Protection Agency, EPA) of evaluation for health risks [25,26] to evaluate the potential non-carcinogenic risk and the probability of excess lifetime cancer risk due to As exposure from O. sinensis consumption. Additionally, Monte-Carlo Simulation (MCS) were employed to analyze the uncertainty. The health risk posed by O. sinensis consumption from exposure to total As, iAs, and i+uAs (iAs and uAs together) were comparatively estimated in this study. These contributions might be useful in efforts to revive the local O. sinensis-dependent economy in the Qinghai–Tibet Plateau and surrounding countries by providing a comprehensive edible hazard evaluation of O. sinensis.

2. Results and Discussion

2.1. Analytical Performances of the Proposed Method

Extraction efficiency for As speciation analysis was assessed based on a comparison between the total As concentration in 0.15 mol/L HNO3 extracts and concentrated HNO3 extracts using a microwave digestion system (see Section 3.2). The concentrations of the total As in the extracts through different extraction methods were quantitively consistent, with an extraction efficiency of 92.3–104% (the ratio of total As concentration in 0.15 mol/L HNO3 extracts to that in concentrated HNO3 extracts). This efficiency indicates that most As species were thoroughly extracted through the current method (see Section 3.2).
The analytical performances of the proposed ICP-MS, for total As content analysis, and HPLC-ICP-MS, for As species analysis, methods were validated by determining the linearity, limits of detection (LOD), and limits of quantification (LOQ) as shown in Table 1. The linear correlation coefficients, in the range of 0.5–500 μg L−1(for total As) and 0.2–300 μg L−1 (for As species), were greater than 0.9997. LOD of total As, AsB, DMAV, AsШ, MMAV, and AsV were 2.3, 1.1, 1.3, 1.0, 2.2, and 1.1 μg kg−1, respectively, And the LOQ were found to be in the range of 3.0–6.9 μg kg−1 for the total As content and the five arsenic species. The recoveries of the total As content were in the range 92.3−106.6% and the relative standard deviations (RSDs) were in the range 2.4~4.6%. The recoveries and RSDs of arsenic species were studied by spiking three concentration levels of the five arsenic species into the O. sinensis samples. The recoveries of arsenic species were in the range 86.3~111.7% and the RSDs were in the range 0.8~6.6%, as shown in Table 2. The analysis results of certified reference materials (CRM) using the same method were in good agreement with their certified values and the recoveries were 97.5–101% for total As analysis and 110.8% for iAs analysis as shown in Table 3.

2.2. Total Arsenic Concentration and Arsenic Species in O. sinensis Samples

The total As content in O. sinensis ranged from 4.00 mg/kg to 5.25 mg/kg of dry mass (Table 4), which is consistent with that in the previous studies [21] that caused uneasiness and fear in consumers. The Chinese government, through the National Health and Family Planning Commission of the People’s Republic of China, established a reference value of 1 mg/kg for total As in functional foods (GB16740-2014) [9]. Thus, the total As content in O. sinensis exceeds the limit of As in functional foods in China (Figure 3). These data demonstrate that there is an urgent need to determine As speciation in O. sinensis samples, because the total amount of As does not provide sufficient toxicological information. In Figure 4B, the large peak area indicates that AsШ, which is generally considered most toxic, might be the major As species in O. sinensis, however, an H2O2 test proved that most of the As is not oxidized to AsV (Figure 4C) and hence the major overlapped peak cannot be the toxic AsШ. Thus, according to current chromatography conditions, an H2O2 test is necessary to minimize misidentification. The iAs, calculated according to the AsV content in Figure 4C, is relatively abundant compared to the total As content, in the range 6.0% to 8.3% (the amount of AsV in Figure 4C), but is small compared with the amount of organic As. Organic As species were predominant, and the percentage of total As content ranged from 91.7% to 94.0%. The two potential toxic organic As species, MMAV and DMAV, were found to be negligible. Because the initial organic metabolites of iAs, MMAV, and DMAV were trace in O. sinensis sample, it can be inferred that they were transformed into other organic arsenic metabolites in the organism such as AsB (which was also detected as a minority compound in these samples, ranging from 1.4% to 2.9%) and various unknown organic As species (uAs, a cluster of unknown compounds, with a retention time from 3.2 to 4 min, representing 89.0% to 92.3% of the of the total As content, Figure 4C). Based on previous studies, comparison of the retention time behavior of standards with that of the extracts upon changing pH of the mobile phase can ascertain the presence of an expected compound or give useful information on the nature of unknown compounds [27]. In this study, despite the lack of standards for the confirmation of uAs, based on the overlap with AsШ, organic As (oAs) species which have low retention under anion exchange HPLC-ICP-MS chromatograms, such as arsenolipids and AsHCs which were usually analyzed using reversed-phase HPLC-ICP-MS, can be excluded [28]. In addition, according to its stability under the H2O2 treatment, unprotected trivalent oAs species which can be oxidized, such as DMAШ, MMAШ, and thio-organoarsenic (DMMTAV), can also be excluded. Thus, due to its chemical characteristics, the uAs in O. sinensis cannot be the toxic oAs which have been discovered so far (Figure 5). The chromatography behavior indicates that it might be an arsenosugar(s), which are frequently reported to be co-eluted with DMA, AsB, MMA, and AsШ under anion exchange HPLC-ICP-MS chromatograms [29].

2.3. Hazard Risk Assessment of Long-Term O. sinensis Consumption

Because the toxicity of As species differs [17], it is especially important to determine the chemical form of As in O. sinensis samples, and a health risk assessment should focus on toxic As species because of their carcinogenic potential rather than the total As content. Based on the As speciation analysis method of the latest Chinese national standard to determine As species in functional food (GB 16740-2014), in this work iAs and AsB were minor As components. Additionally, MMAV and DMAV were negligible, leaving the majority of As content as unknown organic As. Till the end of the 1990s, iAs was assumed as the toxic actor among all the As species, and oAs was assumed less toxic or non-toxic. Thus, according to conventional opinion on the toxicity of As species, the negligible abundances of toxic As in O. sinensis show that the oral intake hazard might be lower than in iAs-accumulated mushrooms, such as Laccaria amethystea [30], Collybia butyracea [31] and other mushrooms [32]. However, this assumption was questioned with the development of analytic methods. Arsenic undergoes rapid and complicated metabolism in organisms, and several organic As species, discovered as intermediate metabolites (MMAШ, DMAШ, DMMTAV) in organisms or discovered in animal-sourced foods, were found to be strongly toxic (AsHCs) [15,16,17,18,19]. In this study, although the traditionally considered toxic As (iAs, DMAV, MMAV) in O. sinensis is minor, the large amount of organic As cannot be accurately determined for its speciation and thus its toxicity cannot be arbitrarily evaluated. In order to comprehensively assess long-term O. sinensis consumption, based on the intake of total As, i+uAs (considering uAs is toxic, as iAs), and iAs (consider uAs is non-toxic, as AsB) the average values of the estimated daily intake (EDI), hazard quotient (HQ), and cancer risk (CR) were calculated according to Equations (1)–(3), respectively.
According to the intake of total As, the average of HQtotal As = 2.6 and CRtotal As = 1.2 × 10−3. A Monte-Carlo simulation was used to model the uncertainties of the EDI, HQ, and CR. After 10,000 iterations of each simulation, EDI, HQ, and CR were derived, and the distribution patterns are illustrated in Figure 6. In terms of the health risk associated with total As exposure, the average EDItotal As (Figure 6A) from O. sinensis consumption is 8.00 × 10−4 mg/(kg·day), the HQtotal As > 1 (Figure 6B), and the mean, median, 5th percentile, and 95th percentile values of CRtotal As are 1.23 × 10−3, 1.20 × 10−3, 8.14 × 10−4, and 1.78 × 10−3, respectively (Figure 6A–C). According to the average value and uncertainty analysis of EDI, HQ, and CR, O. sinensis would not be recommended for long-time consumption, as assessed by the total As content (HQtotal As > 1 and CRtotal As > 1 × 10−4). However, as we discussed before, applying total As content in the hazard risk assessment is unfair; the hazard risk assessment should be based on the concentration of toxic As species. However, the majority of the As species in O. sinensis were unknown using the current five arsenic standards, thus the toxicity is also unknown. Hence, we assess the edible hazard according to two postulated situations. First, if the uAs is as toxic as AsШ, based on the intake of i+uAs, HQi+uAs = 2.62 and CRi+uAs = 1.2 × 10−3. In terms of the health risk associated with i+uAs exposure after the Monte-Carlo simulation, the mean, median, 5th percentile, and 95th percentile values of CRi+uAs are 1.18 × 10−3, 1.18 × 10−3, 1.00 × 10−3, and 1.36 × 10−3, respectively (Figure 6D–F). These results indicate a similar edible hazard as that predicted by the total As analysis. Second, if the uAs is non-toxic as AsB and AsC, the relative abundance of the toxic iAs is only 10% of the total As content, and, and correspondingly, the average value (EDIiAs = 5.95 × 10−5 mg/(kg·day), HQiAs = 0.19 < 1, and CRiAs = 8.7 × 10−5 < 1 × 10−4) and uncertainty analysis of EDI, HQ, and CR were much lower and below the hazardous thresholds. The mean, median, 5th percentile, and 95th percentile values of CRtoxic As were 8.93 × 10−5, 8.66 × 10−5, 5.83 × 10−5, and 1.29 × 10−4, respectively (Figure 6G–I). The CR value exceeded the threshold value (1 × 10−4) at the 72.7th percentile. Thus, if the uAs is non-toxic, the long-term consumption of O. sinensis is relatively safe according to the hazard analysis on iAs and the reputation of O. sinensis should be rehabilitated because of its non-toxicity. As we discussed in Section 2.2, the uAs is might be an arsenosugar, which are generally considered to be non-toxic or have low toxicity [29]. Thus, according to the current recognition on the toxicity of organic As species, the uAs might be non-toxic, and accordingly the long consumption of O. sinensis might be safe in terms of As speciation analysis. However, determination of the structure and toxicity of the uAs should be carried out to provide strong evidence for our inference.
Although As has become notorious because some As species cause acute toxicity, chronic toxicity, and cancer via environmental exposure at low doses, its medicinal properties have been a focus in ancient China and Greece for over 3000 years [13]. Until the late 1940s, diseases such as psoriasis and syphilis were frequently treated with As [33]. More recently, inorganic arsenic has also shown significant activity in curing acute promyelocytic leukemia (APL) [13]. Inorganic arsenic has shown activity against other hematologic and solid organ malignancies but is also associated with serious toxicities, especially when applied at higher doses. For decades, scientists have attempted to seek ideal As compounds that are nontoxic and have medicinal potential. Few efforts have been successful, and the medical use of the most concerning forms of organic As (such as MMAV, DMAV, and AsB) has not been proven yet [33]. The recent development of orally bio-available organic arsenic derivatives (OAD) offering improved toxicity profiles and better efficacy may expand the application of arsenic compounds in hematologic malignancies and solid tumors [33,34]. O. sinensis has been massively reported to have predominant efficiency in anti-tumorigenesis, and most researchers consider this the result of chemical constituents such as cordycepin, adenosine, the exopolysaccharide fraction, cordyglucans, and monosaccharide saponins [5]. From our newly obtained data, inorganic As comprised only 7% of the total As content, much lower than that (90%) of unknown organic As. Inspired by the above-mentioned progress in the medicinal use of organic arsenic derivatives (OAD) [33,34], we infer that the unknown As might play certain important roles in anti-tumorigenesis or other functional uses of O. sinensis. Therefore, combining the risk hazard analysis and potential functional speculation, As might be considered functional in O. sinensis instead of poisonous. Further research to provide sufficient evidence on the above speculation including structural identification, toxicology, and pharmacology assessments, especially animal testing, should be implicated in future.

3. Materials and Methods

3.1. Reagents

Deionized water obtained from a Milli-Q water purification system (Millipore, Bedford, MA, USA) was used for the preparation of all solutions. Anhydrous sodium acetate, potassium nitrate, sodium dihydrogen phosphate, and disodium ethylenediaminetetraacetate (EDTA) were used for the preparation of mobile phases. H2O2 (31%) and concentrated HNO3 (69%) were used for sample digestion and extraction. Standards for the determination of both total As content and As speciation were prepared by diluting or compounding the standard stock solution sourced from the National Institute of Metrology (Beijing, China). The former was diluted to 10 mg As/L from the stock solution (AsV) with certified concentration of 1000 mg As/L, and the latter was prepared with AsШ (0.233 μmol/g), AsV (1.011 μmol/g), MMAV (0.355 μmol/g), DMAV (0.706 μmol/g), and AsB (0.518 μmol/g). All stock solutions were kept at 4 °C, and further dilutions for analysis were prepared daily. National Standard reference materials: GBW10049 (green Chinese onion, Institute of Geophysical and Geochemical Exploration, Langfang, China), GBW10051 (pork liver, Institute of Geophysical and Geochemical Exploration, Langfang, China), GBW08573 (yellow-fin tuna, Second Institute of Oceanography, Huangzhou, China) and GBW(E)100358 (rice, National Analysis Center for Ion and Steel, Beijing, China) were used as certified reference materials (CRMs) in this study.

3.2. Sample Collection and Preparation

Three typical production areas (Litang, Naqu, and Yushu) were chosen as the sampling sites (Figure 2), and twenty O. sinensis samples of similar size (approximately 0.3 g per sample) were collected at each site in May 2017. All the samples were mechanically cleaned of soil, rinsed with deionized water and freeze-dried to reach a constant weight. To gather enough material for subsequent As analysis, each of the five O. sinensis samples was combined into a batch sample. Therefore, the original twenty O. sinensis taken at each sampling site were organized into 4 sample batches (referred to as NQ1–4, LT1–4 and YS1–4). These sample batches were individually pulverized using a mortar and pestle to reduce the particle size. Then, the fine powder was passed through a sieve with a pore size of 0.25 mm and stored in sealable plastic bags at 4 °C until analysis.
For total As content analysis, digestion of approximately 500 mg of sample powder was conducted in a microwave digestion system using concentrated HNO3. The operating program of the microwave system was as follows: the samples were heated to 120 °C in 5 min and held at 120 °C for 5 min in the first step, heated to 150 °C in 5 min and held at 150 °C for 5 min in the second step, heated to 170 °C in 5 min and held at 170 °C for 5 min in the third step, and heated to 190 °C in 5 min and held at 190 °C for 20 min in the fourth step. Finally, the samples were cooled to room temperature. For As speciation analysis, approximately 1 g of sample powder was diluted with 20 mL 0.15 mol/L HNO3 in a 50 mL polyethylene centrifuge tube and then incubated in a 90 °C water bath for 12 h. All the digestion products were centrifuged, filtered, and stored at 4 °C before total As and As speciation analysis.

3.3. Total Arsenic Analysis

The total As content of each sample was measured using ICP-MS (Agilent 7800, Santa Clara, CA, USA). The supernatant was first diluted with water up to 25 mL and then subjected to ICP-MS. The operating parameters of the equipment were as follows: radio frequency (RF) power, 1550 W; carrier gas, 1.05 L/min; collision mode, Helium (HE) flow 4.2 mL/min; plasma gas flow rate = 15 L/min; auxiliary gas flow rate = 0.1 L/min; and selected isotope = m/z 75. Samples were quantified using an external calibration curve from AsV standards (calibration points: 5, 10, 50, 100, and 200 ppm). Triplicate analyses were performed for each sample. For quality control purposes, the standards used for the calibration curve were run before and after each sample series. The corresponding digestion blanks (one for each sample digestion series) were also measured. The total As in the CRMs (GBW10049, GBW10051, GBW08573 and GBW100358) were determined according the same methods.

3.4. Arsenic Speciation Analysis

The separation and determination of As species were performed according to our previous HPLC-ICP-MS method [35]. Five As species (AsШ, AsV, MMAV, DMAV, AsB) were separated by an Agilent 1260 HPLC system (Agilent, USA). The chromatograph was equipped with a standard autosampler, IonPac AG19 guard column (4 × 50 mm), and an IonPacAS19 separation column (4 × 250 mm). The main chromatography conditions used for HPLC were as follows: a mobile phase of 10 mmol/L anhydrous sodium acetate; 3 mmol/L potassium nitrate; 10 mmol/L sodium dihydrogen phosphate; 0.2 mmol/L disodium ethylenediaminetetraacetate buffer; a flow rate at 1.0 mL/min; a column temperature of room temperature; and an injection volume with a 50 μL sample. The separated As species were examined by ICP-MS (as described previously) and identified by comparison of retention times with standards (Figure 4A). External calibration curves (calibration points: 0, 2.5, 5, 10, 50, and 100 ppb) were used to quantify MMAV, DMAV, AsШ, AsV, and AB according to the corresponding standards. Triplicate analyses were performed for each sample. Extraction blanks were also analyzed by HPLC-ICP-MS in each work session.
To check the extraction and digestion efficiency for the As species analysis, the As species extraction was completely measured using ICP-MS according to 3.3. Then the measured total As concentration in the As species extraction which was digested by 0.15 mol/L HNO3, was compared with the total As digested by concentrated HNO3 to calculate the extraction efficiency. The iAs species in one CRM (GBW100358) were determined according the same methods.
Due to the complexity of the metrics of the O. sinensis sample, the AsШ peak could not be separated from the other unknown peaks (Figure 4B); therefore, 1 mL of H2O2 was added to the supernatant before analysis. This operation completely oxidizes AsШ into AsV (Figure 4C), without conversion of other arsenic species into new As compounds, according to the comparison of the number and the area of the corresponding peaks in Figure 4B and C. The inorganic As content was identified and quantified as AsV using external standards. The total organic As amounts were quantified according to the delta value between the total As and the inorganic As content. Thus, the content of the unknown As species was quantified according to the delta value between the organic As and the sum of the three determined As species (MMAV, DMAV, and AsB).

3.5. Health Risk Assessment

The health risks for consumers with exposure to total toxic As (the sum of inorganic As, DMA, and MMAV), total As content, i+uAs, and iAs in O. sinensis were evaluated. According to the model of evaluation for health risks (Environment Protection Agency, EPA) [25,26], the estimated daily intake of As from O. sinensis consumption was calculated according to Equation (1):
E D I = C A s × I R B W ,
where EDI (mg/kg·day) is the estimated daily intake of As, CAs (mg/kg) is the concentration of total As or toxic As in O. sinensis (dry weight), IR (0.01 kg/day) is the daily ingestion rate of O. sinensis [36], and BW is the average body weight (58.7 kg) [37] of the consumer.
Risk characterization was quantified according to the potential non-carcinogenic risks of As exposure from O. sinensis ingestion and was reflected by the hazard quotient (HQ). The HQ value was calculated using equation (2). If HQ < 1, there might be no concern for non-carcinogenic effects. Otherwise, if the HQ exceeds 1, there might be serious concern for non-carcinogenic effects [25].
H Q = ( E D I R f D ) × ( E F × E D A T )
The probability of the excess lifetime cancer risk (CR) due to As exposure for O. sinensis consumption was estimated according to Equation (3). According to the US EPA, the incremental probability of cancer risk for consumers over a lifetime is characterized by CR with an acceptable range of 1 × 10−6–1 × 10−4. A CR less than 1.0 × 10−6 indicates no obvious concern for cancer risk. However, a CR greater than 1.0 × 10−4 indicates an obvious potential cancer risk [25].
C R = E D I × S F × ( E F × E D A T )
In Equations (2) and (3), RfD is the oral reference dose for As, EF (days/year) is the exposure frequency, ED (years) is the exposure duration, AT (days) is the average exposure time to As (usually AT is equal to the average life expectancy), and SF is the slope factor of As through oral intake. In this study, RfD, SF, ED, EF, and AT were determined to be 3 × 10−4 mg/(kg·day) [26], 1.5 mg/(kg·day) [26], 70 years, 365 days/year, and 25,550 days [37], respectively.
To assess the long-term health risks of O. sinensis to arsenic exposure, the uncertainty of EDI, HQ, and CR were modeled through a Monte-Carlo simulation conducted with CrystalBall Software (V. 11.1.2.0.00, Oracle, Redwood Shores, CA, USA). The parameter distributions used in the model are presented in Table A1.

4. Patents

A new method for risk assessment of Ophiocordyceps sinensis. Patent No. 201711087936.2. Document No. 2017110900896410.

Author Contributions

L.-X.G. and Z.-G.H. conceived and designed the experiments; G.-W.Z. and J.-T.W. performed the experiments; Y.-P.Z. and L.-X.G. analyzed the data; Z.-G.H. contributed analysis tools; L.-X.G. wrote the paper.

Acknowledgments

This work was jointly supported by the Excellent College Teachers Training Program of Guangdong Province, China (No. YQ2015084), the Natural Science Foundation of China (No. 81303155), and the Guangdong Provincial Science and Technology Plan Project (No. 2015A020208009).

Conflicts of Interest

The authors declare no conflict of interest. The funding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Abbreviations

O. sinensisOphiocordyceps sinensis
AsArsenic
AsШArsenite
AsVArsenate
DMADimethylarsinic acid
MMAMethylarsonic acid
AsBArsenobetaine
DMMTAThio-organoarsenic
uAsUnknown organic As
oAsOrganic arsenic
iAsInorganic arsenic
i+uAsSum of iAs and uAs
LOQLimits of quantification
LODLimits of detection
EDIEstimated Daily Intake
HQHazard Quotient
CRCancer Risk
HPLC-ICP-MSHigh-Performance Liquid Chromatography–Inductively Coupled Plasma Mass Spectrometry

Appendix A

Table
Table A1. Input parameters for Monte-Carlo simulation.
Table A1. Input parameters for Monte-Carlo simulation.
ParametersUnitsDistribution
Adult Body weight, BWBW kgLognormal (Mean = 58.7, SD = 12.0)
Daily ingestion rate, IRkg/dayLognormal (Mean = 0.01, SD = 0.0143)
Total As concentration, Ctotal Asμg/kgLognormal (Mean = 4.63, SD = 0.42)
i+uAs concentration, Ci+uAsμg/kgLognormal (Mean = 4.53, SD = 0.41)
iAs concentration, CiAsμg/kgLognormal (Mean = 0.34, SD = 0.03)

References

  1. Dong, C.H.; Wen-Jia, L.I. Cordyceps industry in China: Current status, challenges and perspectives–Jinhu declaration for Cordyceps industry development. Mycosystema 2016, 35, 1–15. [Google Scholar] [CrossRef]
  2. Guo, L.X.; Hong, Y.H. Fungus-larva relation in the formation of Cordyceps sinensis as revealed by stable carbon isotope analysis. Sci. Rep. 2017, 7, 7789. [Google Scholar] [CrossRef] [PubMed]
  3. Martel, J.; Ko, Y.F. Myths and realities surrounding the mysterious caterpillar fungus. Trends Biotechnol. 2017, 35, 1017. [Google Scholar] [CrossRef] [PubMed]
  4. Qi, W.; Lei, W. Pharmacological study progress of the Cordyceps sinensis. Global Tradit. Chin. Med. 2014, 7, 227–232. [Google Scholar]
  5. Liu, Y.; Wang, J. The chemical constituents and pharmacological actions of Cordyceps sinensis. Evid. Based Complement. Altern. Med. 2015, 2015, 575063. [Google Scholar]
  6. Caplins, L.; Halvorson, S. Collecting Ophiocordyceps sinensis: An emerging livelihood strategy in the garhwal, indian himalaya. Mt. Sci. 2017, 14, 390–402. [Google Scholar] [CrossRef]
  7. Winkler, D. Yartsa gunbu (Cordyceps sinensis) and the fungal commodification of Tibet’s rural economy. Econ. Bot. 2008, 62, 291–305. [Google Scholar] [CrossRef]
  8. Consumption Tips on Cordyceps sinensis Products. Available online: http://samr.cfda.gov.cn/WS01/CL1986/144020.html (accessed on 4 February 2016).
  9. The Common Standards of Functional Food; GB16740-2014; National Health and Family Planning Commission of the People’s Republic of China: Beijing, China, 2014.
  10. Notice on the Suspension of All the Pilot Work on Cordyceps sinensis for Health Food. Available online: http://samr.cfda.gov.cn/WS01/CL0847/146100.html (accessed on 4 March 2016).
  11. Wang, H.; Shan, Y. The utilization and analysis of present market situation for Cordyceps sinensis. Res. Pract. Chin. Med. 2016, 30, 83–86. [Google Scholar]
  12. Tsuda, T.; Babazono, A. Inorganic arsenic: A dangerous enigma for mankind. Appl. Organomet. Chem. 2010, 6, 309–322. [Google Scholar] [CrossRef]
  13. Khairul, I.; Wang, Q.; Naranmandura, H. Metabolism, toxicity and anticancer activities of arsenic compounds. Oncotarget 2017, 8, 23905–23926. [Google Scholar] [CrossRef] [PubMed]
  14. Cohen, S.M.; Arnold, L.L.; Eldan, M.; Lewis, A.S.; Beck, B.D. Methylated arsenicals: The implications of metabolism and carcinogenicity studies in rodents to human risk assessment. Crit. Rev. Toxicol. 2006, 36, 99–133. [Google Scholar] [CrossRef] [PubMed]
  15. Styblo, M.; Razo, L.M.D. Comparative toxicity of trivalent and pentavalent inorganic and methylated arsenicals in rat and human cells. Arch. Toxicol. 2000, 74, 289. [Google Scholar] [CrossRef] [PubMed]
  16. Huang, C.; Ke, Q. Molecular mechanisms of arsenic carcinogenesis. Mol. Cell. Biochem. 2004, 255, 57–66. [Google Scholar] [CrossRef] [PubMed]
  17. Hughes, M.F. Arsenic toxicity and potential mechanisms of action. Toxicol. Lett. 2002, 133, 1–16. [Google Scholar] [CrossRef]
  18. Hua, N.; Carew, M.W. Comparative toxicity of arsenic metabolites in human bladder cancer EJ-1 cells. Chem. Res. Toxicol. 2011, 24, 1586–1596. [Google Scholar]
  19. Meyer, S.; Matissek, M.; Müller, S.M.; Taleshi, M.S.; Ebert, F.; Francesconi, K.A.; Schwerdtle, T. In vitro toxicological characterisation of three arsenic-containing hydrocarbons. Metallomics 2014, 6, 1023–1033. [Google Scholar] [CrossRef] [PubMed]
  20. Hao, C.; Wang, G. Arsenic speciation analysis of 15 traditional Chinese medicines. Instrum. Anal. 2009, 28, 918–921. [Google Scholar]
  21. Cao, X.; Wang, J. Analysis of arsenic speciation in Cordyceps sinensis in Tibet by HPLC-HG-AFS. Chin. Tradit. Patent Med. 2015, 37, 1985–1989. [Google Scholar]
  22. Gómezariza, J.L.; Sánchezrodas, D. A comparison between ICP-MS and AFS detection for arsenic speciation in environmental samples. Talanta 2000, 51, 257. [Google Scholar] [CrossRef]
  23. Popp, M.; Hann, S. Environmental application of elemental speciation analysis based on liquid or gas chromatography hyphenated to inductively coupled plasma mass spectrometry—A review. Anal. Chim. Acta 2010, 668, 114–129. [Google Scholar] [CrossRef] [PubMed]
  24. Sankararamakrishnan, N.; Mishra, S. A Comprehensive Review on Various Analytical Methods for the Determination of Inorganic and Organic Arsenic in Environmental Samples. In Environmental Contaminants; Springer: Singapore, 2018. [Google Scholar]
  25. U.S. EPA. Risk Assessment Guidance for Superfund (RAGS). In Human Health Evaluation Manual (Part A); EPA Publication: Washington, DC, USA, 1989. [Google Scholar]
  26. U.S. EPA. Integrated Risk Information System (IRIS). In Arsenic, Inorganic (CASRN 7440-38-2)-748; EPA Publication: Washington, DC, USA, 2009. [Google Scholar]
  27. Francesconi, K.A.; Goessler, W.; Panutrakul, S.; Irgolic, K.J. A novel arsenic containing riboside (arsenosugar) in three species of gastropod. Sci. Total Environ. 1998, 221, 139–148. [Google Scholar] [CrossRef]
  28. Sele, V.; Sloth, J.J.; Holmelid, B.; Valdersnes, S.; Skov, K.; Amlund, H. Arsenic-containing fatty acids and hydrocarbons in marine oils—Determination using reversed-phase HPLC-ICP-MS and HPLC-qTOF-MS. Talanta 2014, 121, 89–96. [Google Scholar] [CrossRef] [PubMed]
  29. Niegel, C.; Matysik, F.M. Analytical methods for the determination of arsenosugars—A review of recent trends and developments. Anal. Chim. Acta 2010, 657, 83. [Google Scholar] [CrossRef] [PubMed]
  30. Larsen, E.; Hansen, M. Speciation and health risk considerations of arsenic in the edible mushroom Laccaria amethystina collected from contaminated and uncontaminated locations. Appl. Organomet. Chem. 1998, 12, 285–291. [Google Scholar] [CrossRef]
  31. Kuehnelt, D.; Goessler, W. Arsenic compounds in terrestrial organisms I: Collybiamaculata, Collybiabutyracea and Amanita muscaria from arsenic smelter sites in Austria. Appl. Organomet. Chem. 1997, 11, 289–296. [Google Scholar] [CrossRef]
  32. Chen, S.; Guo, Q. Determination of arsenic species in Edible mushrooms by high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry. Food Anal. Methods 2017, 10, 740–748. [Google Scholar] [CrossRef]
  33. Quintás-Cardama, A.; Verstovsek, S. Chemical and clinical development of darinaparsin, a novel organic arsenic derivative. Anti-Cancer Agents Med. Chem. 2008, 8, 904. [Google Scholar] [CrossRef]
  34. Kuntze, A.M.; Braz, O. Development of an organic arsenic derivative as a therapy for leukaemia. Spies Int. Symp. Opt. Sci. 2003, 2003, 347–355. [Google Scholar]
  35. Tang, L.; Zhang, L.L. Arsenic speciation of dried Lentinus edodes by HPLC-ICP-MS. Food Ind. 2015, 36, 275–277. [Google Scholar]
  36. Feng-Lei, X.U.; Wen-Ying, H. Clinical efficacy of Cordyceps sinensis for chronic kidney diseases: A systematic review. Chin. J. Evid. Based Med. 2006, 6, 804–808. [Google Scholar]
  37. Ma, L.; Yang, Z.; Kong, Q.; Wang, L. Extraction and determination of arsenic species in leafy vegetables: Method development and application. Food Chem. 2017, 217, 524–530. [Google Scholar] [CrossRef] [PubMed]
Sample Availability: Samples of the compounds AsB, DMA, MMA, AsIII, AsV and uAs are available from the authors.
Molecules 23 01012 g001 550
Figure 1. Life history of Ophiocordyceps sinensis (O. sinensis). (a) The eggs of the host Thitarodes insect, which are scattered on the grassland, incubate; (b) The host larvae safely reside in the soil throughout the long-lasting larval stage; (c) The ascospores erupt from mature fruiting bodies of O. sinensis; (d) The 4–5th instar larvae may be infected by the infective conidia of the O. sinensis fungus in the soil; (e) The caterpillar-shaped sclerotium (winter-worm) is formed; (f) The stroma germinates out of the head capsule and the mature O. sinensis (summer-grass-winter-worm) is formed.
Figure 1. Life history of Ophiocordyceps sinensis (O. sinensis). (a) The eggs of the host Thitarodes insect, which are scattered on the grassland, incubate; (b) The host larvae safely reside in the soil throughout the long-lasting larval stage; (c) The ascospores erupt from mature fruiting bodies of O. sinensis; (d) The 4–5th instar larvae may be infected by the infective conidia of the O. sinensis fungus in the soil; (e) The caterpillar-shaped sclerotium (winter-worm) is formed; (f) The stroma germinates out of the head capsule and the mature O. sinensis (summer-grass-winter-worm) is formed.
Molecules 23 01012 g001
Molecules 23 01012 g002 550
Figure 2. The producing area of Ophiocordyceps sinensis in China and sampling details of this study. (A) Schematic map illustrating the sampling sites in the Qinghai–Tibetan Plateau and its adjacent high-altitude areas. Litang (LT), Naqu (NQ), and Yushu (YS) were chosen as the sampling sites. (B) The grassland of the O. sinensis habitat. Pastoralists are encamped there to collect it. (C) The stroma (a) of O. sinensis emerged out of the ground. (D) O. sinensis in the soil, the yellow arrows pointing out its sclerotium (b) and stroma (c).
Figure 2. The producing area of Ophiocordyceps sinensis in China and sampling details of this study. (A) Schematic map illustrating the sampling sites in the Qinghai–Tibetan Plateau and its adjacent high-altitude areas. Litang (LT), Naqu (NQ), and Yushu (YS) were chosen as the sampling sites. (B) The grassland of the O. sinensis habitat. Pastoralists are encamped there to collect it. (C) The stroma (a) of O. sinensis emerged out of the ground. (D) O. sinensis in the soil, the yellow arrows pointing out its sclerotium (b) and stroma (c).
Molecules 23 01012 g002
Molecules 23 01012 g003 550
Figure 3. The concentration (mg/kg dry weight) of total As and As speciation detected in O. sinensis. Inorganic As ( Molecules 23 01012 i001 AsШ and Molecules 23 01012 i002 AsV) are shown in red and yellow sections, and organic As ( Molecules 23 01012 i003 AsB and Molecules 23 01012 i004 uAs) is shown in dark and light gray sections.
Figure 3. The concentration (mg/kg dry weight) of total As and As speciation detected in O. sinensis. Inorganic As ( Molecules 23 01012 i001 AsШ and Molecules 23 01012 i002 AsV) are shown in red and yellow sections, and organic As ( Molecules 23 01012 i003 AsB and Molecules 23 01012 i004 uAs) is shown in dark and light gray sections.
Molecules 23 01012 g003
Molecules 23 01012 g004 550
Figure 4. Chromatograms obtained in quantification by HPLC-ICP-MS. (A) A mix of standard samples of AsB, DMAV, AsШ, MMA, and AsV, at 10 ppb of each arsenic species. (B) The extracts of sample NQ1. The AsШ and unknown organic As peaks overlap. (C) Oxidation products of the extracts of NQ1. Any AsШ is transformed into AsV when H2O2 is added to the extracts.
Figure 4. Chromatograms obtained in quantification by HPLC-ICP-MS. (A) A mix of standard samples of AsB, DMAV, AsШ, MMA, and AsV, at 10 ppb of each arsenic species. (B) The extracts of sample NQ1. The AsШ and unknown organic As peaks overlap. (C) Oxidation products of the extracts of NQ1. Any AsШ is transformed into AsV when H2O2 is added to the extracts.
Molecules 23 01012 g004
Molecules 23 01012 g005 550
Figure 5. Inference on the toxicity of the unknown As detected in O. sinensis. (a) 1 mL of H2O2 was added to the extracts, and the unknown As could not be oxidized (Figure 4C). Thus, the unknown As cannot be the toxic MMAШ, DMAШ, or DMMTAV which can be oxidized under treatment with H2O2. (b) The unknown peak presents a similar retention time with AsШ, indicating that it is not a low retention component, such as an As hydrocarbon (AsHC).
Figure 5. Inference on the toxicity of the unknown As detected in O. sinensis. (a) 1 mL of H2O2 was added to the extracts, and the unknown As could not be oxidized (Figure 4C). Thus, the unknown As cannot be the toxic MMAШ, DMAШ, or DMMTAV which can be oxidized under treatment with H2O2. (b) The unknown peak presents a similar retention time with AsШ, indicating that it is not a low retention component, such as an As hydrocarbon (AsHC).
Molecules 23 01012 g005
Molecules 23 01012 g006 550
Figure 6. Estimated distribution patterns and descriptive statistics of (A,D,G) estimated daily intake (EDI), (B,E,H) hazard quotient (HQ), and (C,F,I) cancer risk (CR). Where in (A–C) values were derived according to the total As concentration in O. sinensis; in (D–F) values were derived according to the i+uAs concentration (the sum of iAs and uAs) in O. sinensis; and in (G–I) values were derived according to the iAs concentration in O. sinensis.
Figure 6. Estimated distribution patterns and descriptive statistics of (A,D,G) estimated daily intake (EDI), (B,E,H) hazard quotient (HQ), and (C,F,I) cancer risk (CR). Where in (A–C) values were derived according to the total As concentration in O. sinensis; in (D–F) values were derived according to the i+uAs concentration (the sum of iAs and uAs) in O. sinensis; and in (G–I) values were derived according to the iAs concentration in O. sinensis.
Molecules 23 01012 g006
Table
Table 1. Analytical performance of inductively coupled plasma mass spectrometry (ICP-MS) for total arsenic content and HPLC-ICP-MS for arsenic species.
Table 1. Analytical performance of inductively coupled plasma mass spectrometry (ICP-MS) for total arsenic content and HPLC-ICP-MS for arsenic species.
AnalytesLinear Range (μg/L)Linear EquationRLOD (μg/kg)LOQ (μg/kg)
Total As0.5–500y = 2731.03x + 5.55670.99982.36.9
AsB0.2–300y = 18,867.9x + 2319.60.99991.13.3
DMAV0.2–300y = 19,212.4x + 4065.20.99991.34.0
AsШ0.2–300y = 17,090.6x + 3289.10.99971.03.0
MMAV0.5–300y = 18,373.6x + 196.81.00002.26.6
AsV0.2–300y = 18,504.6x + 8247.21.00001.13.3
Table
Table 2. Recovery and precision of the methods.
Table 2. Recovery and precision of the methods.
AnalytesBackground Value (mg/kg)Added (μg/L)Measured Value (μg/L)Recovery (%)RSD (%, n = 6)
Total As0.515.0014.8~15.392.3~99.44.6
10.020.4~21.594.7~102.33.8
50.060.7~64.395.8~106.62.4
AsB0.0102.002.09~2.2691.7~100.14.2
10.09.45~9.8791.9~96.21.9
50.042.0~47.083.6~93.45.0
DMAVND2.001.73~1.9886.5~99.05.7
10.09.20~9.5592.0~95.51.5
50.050.4~52.6100.9~105.21.7
AsШ1.702.0045.3~45.686.3~99.25.5
10.052.8~53.788.8~98.04.3
50.096.8~99.2106.9~111.72.0
MMAV0.00312.002.07~2.1999.4~105.72.7
10.010.6~10.8105.0~107.20.85
50.047.2~48.694.2~97.11.2
AsV0.122.004.81~5.0886.6~100.06.6
10.012.8~13.097.4~99.40.80
50.049.0~50.291.9~94.41.1
Table
Table 3. National standard reference materials values (mg/kg, mean ± standard deviation) and determined values for total As and inorganic arsenic (iAs) content (n = 5).
Table 3. National standard reference materials values (mg/kg, mean ± standard deviation) and determined values for total As and inorganic arsenic (iAs) content (n = 5).
Sample TypeReference MaterialsCertified Value (mg/kg)Determined Value (mg/kg)Recovery (%)
Green Chinese onionGBW100490.52 ± 0.110.507 ± 0.0897.5
Pork liverGBW100511.4 ± 0.31.42 ± 0.15101.4
Yellow-fin tunaGBW085735.08 ± 0.394.98 ± 0.1198.0
RiceGBW1003580.16 ± 0.02 (total As)0.165 ± 0.012103.1
0.13 ± 0.02 (iAs)0.144 ± 0.006110.8
Table
Table 4. Concentration a of total arsenic and arsenic species in Ophiocordyceps sinensis.
Table 4. Concentration a of total arsenic and arsenic species in Ophiocordyceps sinensis.
Sample NameAsB b mg/kg (%)DMAVMMAVuAs mg/kg (%)AsШ mg/kg (%)AsV mg/kg (%)iAs mg/kg (%)oAs mg/kg (%)Total As mg/kg
NQ10.10 (2.1%)nd cnd4.34 (91.2%)0.22 (4.6%)0.10 (2.1%)0.32 (6.7%)4.44 (93.3%)4.76
NQ20.09 (1.8%)ndnd4.59 (91.8%)0.21 (4.2%)0.11 (2.2%)0.32 (6.4%)4.68 (93.6%)5.00
NQ30.12 (2.3%)ndnd4.81(91.6%)0.25 (4.8%)0.07 (1.3%)0.32 (6.1%)4.93 (93.9%)5.25
NQ40.08 (2.0%)ndnd3.69 (90.0%)0.23 (5.6%)0.10 (2.4%)0.33 (8.0%)3.77 (92.0%)4.10
LT10.07 (1.7%)ndnd3.69 (90.2%)0.20 (4.9%)0.13 (3.2%)0.33 (8.1%)3.76 (91.9%)4.09
LT20.09 (2.2%)ndnd3.72 (89.6%)0.22 (5.3%)0.12 (2.9%)0.34 (8.2%)3.81 (91.8%)4.15
LT30.11 (2.8%)ndnd3.56 (89.0%)0.24 (6.0%)0.09 (2.3%)0.33 (8.3%)3.67 (91.7%)4.00
LT40.09 (1.7%)ndnd4.75 (92.2%)0.21 (4.1%)0.10 (1.9%)0.31 (6.0%)4.84 (94.0%)5.15
YS10.12 (2.6%)ndnd4.19 (89.3%)0.27 (5.8%)0.11 (2.3%)0.38 (8.1%)4.31 (91.9%)4.69
YS20.08 (1.9%)ndnd3.80 (90.5%)0.24 (5.7%)0.08 (1.9%)0.32 (7.6%)3.88 (92.4%)4.20
YS30.07 (1.4%)ndnd4.69 (92.3%)0.25 (4.9%)0.07 (1.4%)0.32 (6.3%)4.76 (93.7%)5.08
YS40.15 (2.9%)ndnd4.65 (90.5%)0.22 (4.3%)0.12 (2.3%)0.34 (6.6%)4.8 (93.4%)5.14
AVR d0.09 (1.9%)ndnd4.21 (90.9%)0.23 (5.0%)0.10 (2.2%)0.33 (7.1%)4.3 (92.9%)4.63
a Concentrations are presented as the average value of three measurements with a relative standard deviation (RSD) of less than 8% (the ranges of RSD values were as follows, RSDAsB: 2.1~5.6%, RSDDMAV: 1.8~6.1%, RSDAsШ: 2.0~6.7%, RSDMMAV: 1.4~5.2%, and RSDAsV: 1.1~7.3%; b AsB, MMA, DMA, uAs, AsⅢ, AsⅤ, iAs, oAs, and Total As were the abbreviation of arsenobetaine, monomethylarsonic acid, dimethylarsenic acid, unknown organic arsenic, arsenite, and arsenate, inorganic arsenic (total), organic arsenic (total), and total arsenic, respectively. c not detected; d average value among all the samples.

Share and Cite

MDPI and ACS Style

Guo, L.-X.; Zhang, G.-W.; Wang, J.-T.; Zhong, Y.-P.; Huang, Z.-G. Determination of Arsenic Species in Ophiocordyceps sinensis from Major Habitats in China by HPLC-ICP-MS and the Edible Hazard Assessment. Molecules 2018, 23, 1012. https://doi.org/10.3390/molecules23051012

AMA Style

Guo L-X, Zhang G-W, Wang J-T, Zhong Y-P, Huang Z-G. Determination of Arsenic Species in Ophiocordyceps sinensis from Major Habitats in China by HPLC-ICP-MS and the Edible Hazard Assessment. Molecules. 2018; 23(5):1012. https://doi.org/10.3390/molecules23051012

Chicago/Turabian Style

Guo, Lian-Xian, Gui-Wei Zhang, Jia-Ting Wang, Yue-Ping Zhong, and Zhi-Gang Huang. 2018. "Determination of Arsenic Species in Ophiocordyceps sinensis from Major Habitats in China by HPLC-ICP-MS and the Edible Hazard Assessment" Molecules 23, no. 5: 1012. https://doi.org/10.3390/molecules23051012

Article Metrics

Back to TopTop