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Molecules 2018, 23(5), 1013; https://doi.org/10.3390/molecules23051013

Identification of Ophiocordyceps sinensis and Its Artificially Cultured Ophiocordyceps Mycelia by Ultra-Performance Liquid Chromatography/Orbitrap Fusion Mass Spectrometry and Chemometrics

1
School of Chinese Material Medica, Beijing University of Chinese Medicine, No. 11Beisanhuan east Road, Beijing 100029, China
2
Research and Inspection Center of Traditional Chinese Medicine and Ethnomedicine, National Institutes for Food and Drug Control, State Food and Drug Administration, No. 2 TiantanXili, Beijing 100050, China
3
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, No. 1 Second north Road of zhongguancun, Beijing 100190, China
4
Institute of Microbiology, Chinese Academy of Sciences, No. 1 West Beichen Road, Beijing 100101, China
*
Authors to whom correspondence should be addressed.
Received: 25 March 2018 / Revised: 18 April 2018 / Accepted: 19 April 2018 / Published: 26 April 2018
(This article belongs to the Special Issue Mass Spectrometric Proteomics)
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Abstract

Since the cost of Ophiocordyceps sinensis, an important fungal drug used in Chinese medicine, has increased dramatically, and the counterfeits may have adverse health effects, a rapid and precise marker using the peptide mass spectrometry identification system could significantly enhance the regulatory capacity. In this study, we determined the marker peptides in the digested mixtures of fungal proteins in wild O. sinensis fruiting bodies and various commercially available mycelium fermented powders using ultra-performance liquid chromatography/Orbitrap Fusion mass spectrometry coupled with chemometrics. The results indicated the following marker peptides: TLLEAIDSIEPPK (m/z 713.39) was identified in the wild O. sinensis fruiting body, AVLSDAITLVR (m/z 579.34) was detected in the fermented O. sinensis mycelium powder, FAELLEK (m/z 849.47) was found in the fermented Ophiocordyceps mycelium powder, LESVVTSFTK (m/z 555.80) was discovered in the artificial Ophiocordyceps mycelium powder, and VPSSAVLR (m/z 414.75) was observed in O. mortierella mycelium powder. In order to verify the specificity and applicability of the method, the five marker peptides were synthesized and tested on all samples. All in all, to the best of our knowledge, this is the first time that mass spectrometry has been employed to detect the marker peptides of O.sinensis and its related products. View Full-Text
Keywords: Ophiocordyceps sinensis; ultra-performance liquid chromatography/Orbitrap Fusion mass spectrometry; chemometrics; fungi marker peptide; quality control Ophiocordyceps sinensis; ultra-performance liquid chromatography/Orbitrap Fusion mass spectrometry; chemometrics; fungi marker peptide; quality control
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Zhang, P.; Li, S.; Li, J.; Wei, F.; Cheng, X.; Zhang, G.; Ma, S.; Liu, B. Identification of Ophiocordyceps sinensis and Its Artificially Cultured Ophiocordyceps Mycelia by Ultra-Performance Liquid Chromatography/Orbitrap Fusion Mass Spectrometry and Chemometrics. Molecules 2018, 23, 1013.

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