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Molecules 2018, 23(4), 936; https://doi.org/10.3390/molecules23040936

Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell Detection

1
Department of Chemistry, Boston University, Boston, MA 02215, USA
2
Departments of Biomedical Engineering and Medicine, Boston University, Boston, MA 02215, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: Ashok Kakkar
Received: 21 March 2018 / Revised: 10 April 2018 / Accepted: 11 April 2018 / Published: 18 April 2018
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Abstract

Hydrogels are of keen interest for a wide range of medical and biotechnological applications including as 3D substrate structures for the detection of proteins, nucleic acids, and cells. Hydrogel parameters such as polymer wt % and crosslink density are typically altered for a specific application; now, fluorescence can be incorporated into such criteria by specific macromonomer selection. Intrinsic fluorescence was observed at λmax 445 nm from hydrogels polymerized from lysine and aldehyde- terminated poly(ethylene glycol) macromonomers upon excitation with visible light. The hydrogel’s photochemical properties are consistent with formation of a nitrone functionality. Printed hydrogels of 150 μm were used to detect individual cell adherence via a decreased in fluorescence. The use of such intrinsically fluorescent hydrogels as a platform for cell sorting and detection expands the current repertoire of tools available. View Full-Text
Keywords: dendrimer; hydrogel; screening; crosslinking; fluorescence dendrimer; hydrogel; screening; crosslinking; fluorescence
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Christadore, L.; Grinstaff, M.W.; Schaus, S.E. Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell Detection. Molecules 2018, 23, 936.

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