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Molecules 2018, 23(4), 769; https://doi.org/10.3390/molecules23040769

Charge-Controlled Synthetic Hyaluronan-Based Cell Matrices

1
Department of Cellular Biophysics, Max Planck Institute for Medical Research, 69120 Heidelberg, Germany
2
Department of Biophysical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany
3
Chemistry Department, University of Stuttgart, 70569 Stuttgart, Germany
Present address: Department of Biochemistry, Smith College Northampton, Northampton, MA 01063, USA.
*
Author to whom correspondence should be addressed.
Academic Editor: Silvia Arpicco
Received: 21 February 2018 / Revised: 22 March 2018 / Accepted: 23 March 2018 / Published: 27 March 2018
(This article belongs to the Special Issue Hyaluronic Acid and its Derivatives for Biomedical Applications)
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Abstract

The extracellular matrix (ECM) represents a highly charged and hydrated network in which different cells in vertebrate tissues are embedded. Hydrogels as minimal ECM mimetics with a controlled chemistry offer the opportunity to vary material properties by varying the negative network charge. In this paper, a synthetic biology model of the ECM based on natural and highly negatively charged polyelectrolyte hyaluronic acid (HA) is characterized with specific emphasis on its charge-related bioactivity. Therefore, the thiol-Michael addition click reaction is used to produce HA hydrogels with defined network structure and charge density. The presented hydrogels show enzymatic degradability and cell attachment. These properties depend on both covalent and electrostatic interactions within the hydrogel network. Furthermore, no unspecific or specific attachment of proteins to the presented hydrogels is observed. In addition, these fundamental insights into charge-related ECM behavior and the influence of electrostatic properties could also lead to innovations in existing biomedical products. View Full-Text
Keywords: hyaluronan; polyelectrolyte hydrogel; enzymatic degradation; synthetic ECM; cell attachment; glycosaminoglycans; tissue engineering hyaluronan; polyelectrolyte hydrogel; enzymatic degradation; synthetic ECM; cell attachment; glycosaminoglycans; tissue engineering
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Hegger, P.S.; Kupka, J.; Minsky, B.B.; Laschat, S.; Boehm, H. Charge-Controlled Synthetic Hyaluronan-Based Cell Matrices. Molecules 2018, 23, 769.

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