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Molecules 2018, 23(3), 623; https://doi.org/10.3390/molecules23030623

Efficient Separation of Four Antibacterial Diterpenes from the Roots of Salvia Prattii Using Non-Aqueous Hydrophilic Solid-Phase Extraction Followed by Preparative High-Performance Liquid Chromatography

1,2,†
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1,2,†
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1,3
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1,2
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1,2
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1,2
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1,2
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1,2
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1,2
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1,2,* and 1,2,*
1
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China
2
Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Xining 810008, China
3
Shaanxi Key Laboratory of Bio-Resources, Shaanxi University of Technology, Hanzhong 723000, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 15 January 2018 / Revised: 7 March 2018 / Accepted: 9 March 2018 / Published: 9 March 2018
(This article belongs to the Special Issue Solid Phase Extraction: State of the Art and Future Perspectives)
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Abstract

An efficient preparative procedure for the separation of four antibacterial diterpenes from a Salvia prattii crude diterpenes-rich sample was developed. Firstly, the XION hydrophilic stationary phase was chosen to separate the antibacterial crude diterpenes-rich sample (18.0 g) into three fractions with a recovery of 46.1%. Then, the antibacterial fractions I (200 mg), II (200 mg), and III (150 g) were separated by the Megress C18 preparative column, and compounds tanshinone IIA (80.0 mg), salvinolone (62.0 mg), cryptotanshinone (70.0 mg), and ferruginol (68.0 mg) were produced with purities greater than 98%. The procedure achieved large-scale preparation of the four diterpenes with high purity, and it could act as a reference for the efficient preparation of active diterpenes from other plant extracts. View Full-Text
Keywords: Salvia prattii; antibacterial diterpenes; hydrophilic solid-phase extraction; preparative high-performance liquid chromatography Salvia prattii; antibacterial diterpenes; hydrophilic solid-phase extraction; preparative high-performance liquid chromatography
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Dang, J.; Cui, Y.; Pei, J.; Yue, H.; Liu, Z.; Wang, W.; Jiao, L.; Mei, L.; Wang, Q.; Tao, Y.; Shao, Y. Efficient Separation of Four Antibacterial Diterpenes from the Roots of Salvia Prattii Using Non-Aqueous Hydrophilic Solid-Phase Extraction Followed by Preparative High-Performance Liquid Chromatography. Molecules 2018, 23, 623.

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