Simultaneous Separation of Eight Lignans in Forsythia suspensa by β-Cyclodextrin-Modified Capillary Zone Electrophoresis
Abstract
:1. Introduction
2. Results and Discussion
2.1. Development of Separation Method
2.1.1. Effect of pH
2.1.2. Effect of Borax Concentrations
2.1.3. Effect of β-CD
2.1.4. Effect of Organic Modifiers
2.2. Method Validation
2.3. Application and Recovery
3. Materials and Methods
3.1. Chemicals and Regents
3.2. Electrophoretic Procedures
3.3. Standard Solutions Preparations
3.4. Sample Preparations
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
No. b | Regression Equation c | Linear Range (μg/mL) | LOD (μg/mL) | LOQ (μg/mL) | d R2 |
---|---|---|---|---|---|
1 | y = 0.0094x − 0.117 | 3.28–131.25 | 0.94 | 3.28 | 0.9978 |
2 | y = 0.0124x − 0.0963 | 3.75–150.00 | 1.07 | 3.75 | 0.9962 |
3 | y = 0.0094x − 0.1055 | 4.19–167.50 | 1.20 | 4.19 | 0.9965 |
4 | y = 0.0092x − 0.0868 | 3.69–147.50 | 1.05 | 3.69 | 0.9919 |
5 | y = 0.0185x − 0.1889 | 3.50–140.00 | 1.00 | 3.50 | 0.9944 |
6 | y = 0.0199x − 0.3224 | 4.38–175.00 | 1.25 | 4.38 | 0.9963 |
7 | y = 0.0134x − 0.1248 | 3.72–148.75 | 1.06 | 3.72 | 0.9945 |
8 | y = 0.0091x − 0.1021 | 3.00–120.00 | 0.86 | 3.00 | 0.9953 |
No. | Types | Contents (μg/g) | |||||
---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 8 | ||
S1 | CE a | 3766 ± 135 | 856 ± 20 | 2618 ± 73 | 6393 ± 103 | 3528 ± 139 | 1894 ± 75 |
S2 | CE a | 3942 ± 151 | 1693 ± 34 | 4039 ± 106 | 8972 ± 187 | 6603 ± 103 | 2904 ± 75 |
S3 | CE a | 36,887 ± 790 | 4707 ± 109 | 26,681 ± 344 | 70,652 ± 978 | 47,686 ± 935 | 20,869 ± 326 |
S4 | CE a | 35,529 ± 800 | 8825 ± 232 | 28,492 ± 721 | 71,299 ± 1023 | 67,462 ± 1013 | 18,304 ± 289 |
S5 | Fruits b | 2009 ± 70 | 506 ± 15 | 1719 ± 42 | 4942 ± 74 | 1594 ± 78 | 1425 ± 58 |
S6 | Fruits b | 1137 ± 20 | 517 ± 14 | 779 ± 11 | 1783 ± 54 | 761 ± 14 | 1104 ± 25 |
S7 | Fruits b | 1110 ± 24 | 606 ± 17 | 1022 ± 27 | 1985 ± 89 | 790 ± 29 | 3497 ± 167 |
S8 | Fruits b | - | 983 ± 24 | - | 611 ± 10 | 491 ± 12 | 793 ± 15 |
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Liang, J.; Gong, F.-Q.; Sun, H.-M. Simultaneous Separation of Eight Lignans in Forsythia suspensa by β-Cyclodextrin-Modified Capillary Zone Electrophoresis. Molecules 2018, 23, 514. https://doi.org/10.3390/molecules23030514
Liang J, Gong F-Q, Sun H-M. Simultaneous Separation of Eight Lignans in Forsythia suspensa by β-Cyclodextrin-Modified Capillary Zone Electrophoresis. Molecules. 2018; 23(3):514. https://doi.org/10.3390/molecules23030514
Chicago/Turabian StyleLiang, Jun, Feng-Qiu Gong, and Hui-Min Sun. 2018. "Simultaneous Separation of Eight Lignans in Forsythia suspensa by β-Cyclodextrin-Modified Capillary Zone Electrophoresis" Molecules 23, no. 3: 514. https://doi.org/10.3390/molecules23030514