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Molecules 2018, 23(2), 463; https://doi.org/10.3390/molecules23020463

Qualitative and Quantitative Phytochemical Analysis of Different Extracts from Thymus algeriensis Aerial Parts

1
Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Université Frères Mentouri, Constantine 1, Route d’Aïn El Bey, 25000 Constantine, Algérie
2
Department of Pharmacy, University “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy
3
Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, P.le Aldo Moro 5, 00185 Rome, Italy
4
Dipartimento di Medicina Sperimentale, Sapienza Università di Roma, P.le Aldo Moro 5, 00185 Rome, Italy
5
Facoltà di Bioscienze e tecnologie agro-alimentari e ambientali, Università di Teramo, Via Renato Balzarini 1, 64100 Teramo, Italy
*
Author to whom correspondence should be addressed.
Received: 15 January 2018 / Revised: 9 February 2018 / Accepted: 17 February 2018 / Published: 20 February 2018
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Abstract

This study was performed to evaluate the metabolite recovery from different extraction methods applied to Thymus algeriensis aerial parts. A high-performance liquid chromatographic method using photodiode array detector with gradient elution has been developed and validated for the simultaneous estimation of different phenolic compounds in the extracts and in their corresponding purified fractions. The experimental results show that microwave-assisted aqueous extraction for 15 min at 100 °C gave the most phenolics-enriched extract, reducing extraction time without degradation effects on bioactives. Sixteen compounds were identified in this extract, 11 phenolic compounds and five flavonoids, all known for their biological activities. Color analysis and determination of chlorophylls and carotenoids implemented the knowledge of the chemical profile of this plant. View Full-Text
Keywords: color analysis; pigments; MAE; HPLC-PDA; SFE; Thymus algeriensis color analysis; pigments; MAE; HPLC-PDA; SFE; Thymus algeriensis
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Boutaoui, N.; Zaiter, L.; Benayache, F.; Benayache, S.; Carradori, S.; Cesa, S.; Giusti, A.M.; Campestre, C.; Menghini, L.; Innosa, D.; Locatelli, M. Qualitative and Quantitative Phytochemical Analysis of Different Extracts from Thymus algeriensis Aerial Parts. Molecules 2018, 23, 463.

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