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Molecules 2018, 23(2), 274; https://doi.org/10.3390/molecules23020274

Rapid Characterization and Identification of Non-Diterpenoid Constituents in Tinospora sinensis by HPLC-LTQ-Orbitrap MSn

1
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China
2
Beijing Research Institution of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
*
Author to whom correspondence should be addressed.
Received: 12 December 2017 / Revised: 23 January 2018 / Accepted: 24 January 2018 / Published: 29 January 2018
(This article belongs to the Collection Herbal Medicine Research)
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Abstract

Tinospora sinensis, a kind of Chinese folk medicine, has functions of harmonizing qi and blood, dredging the channels and collaterals, calming and soothing the nerves. In the present study, a method based on high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (HPLC-LTQ-Orbitrap) was developed for the systematical characterization of the non-diterpenoid constituents which possessed remarkable biological activities in T. sinensis, like anti-tumor, anti-inflammatory, hypoglycemic activity and immunomodulatory activity. Based on the accurate mass measurement (<5 ppm), retention times and MS fragmentation ions, 60 non-diterpenoid constituents were unambiguously or tentatively characterized from T. sinensis extract, including 27 alkaloids, 23 phenylpropanoids, seven sesquiterpenoids and three other constituents. Among them, 13 compounds were tentatively identified as new compounds. Finally, three of the non-diterpenoid constituents were purified and identified, which further confirmed the validity of the results. This study demonstrated that the HPLC-LTQ-Orbitrap MSn platform was a useful and efficient analytical tool to screen and identify constituents in natural medicine. View Full-Text
Keywords: HPLC-LTQ-Orbitrap; non-diterpenoids; fragmentation pathway; Tinospora sinensis HPLC-LTQ-Orbitrap; non-diterpenoids; fragmentation pathway; Tinospora sinensis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Jiao, Q.-S.; Xu, L.-L.; Zhang, J.-Y.; Wang, Z.-J.; Jiang, Y.-Y.; Liu, B. Rapid Characterization and Identification of Non-Diterpenoid Constituents in Tinospora sinensis by HPLC-LTQ-Orbitrap MSn. Molecules 2018, 23, 274.

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