Nigella damascena L. Essential Oil—A Valuable Source of β-Elemene for Antimicrobial Testing
AbstractThe most commonly used plant source of β-elemene is Curcuma wenyujin Y. H. Chen & C. Ling (syn. of Curcuma aromatic Salisb.) with its content in supercritical CO2 extract up to 27.83%. However, the other rich source of this compound is Nigella damascena L. essential oil, in which β-elemene accounts for 47%. In this work, the effective protocol for preparative isolation of β-elemene from a new source—N. damascena essential oil—using high performance counter-current chromatography HPCCC was elaborated. Furthermore, since sesquiterpens are known as potent antimicrobials, the need for finding new agents designed to combat multi-drug resistant strains was addressed and the purified target compound and the essential oil were tested for its activity against a panel of Gram-positive and Gram-negative bacteria, fungi, and mycobacterial strains. The application of the mixture of petroleum ether, acetonitrile, and acetone in the ratio 2:1.5:0.5 (v/v) in the reversed phase mode yielded β-elemene with high purity in 70 min. The results obtained for antimicrobial assay clearly indicated that N. damascena essential oil and isolated β-elemene exert action against Mycobacterium tuberculosis strain H37Ra. View Full-Text
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Sieniawska, E.; Sawicki, R.; Golus, J.; Swatko-Ossor, M.; Ginalska, G.; Skalicka-Wozniak, K. Nigella damascena L. Essential Oil—A Valuable Source of β-Elemene for Antimicrobial Testing. Molecules 2018, 23, 256.
Sieniawska E, Sawicki R, Golus J, Swatko-Ossor M, Ginalska G, Skalicka-Wozniak K. Nigella damascena L. Essential Oil—A Valuable Source of β-Elemene for Antimicrobial Testing. Molecules. 2018; 23(2):256.Chicago/Turabian Style
Sieniawska, Elwira; Sawicki, Rafal; Golus, Joanna; Swatko-Ossor, Marta; Ginalska, Grazyna; Skalicka-Wozniak, Krystyna. 2018. "Nigella damascena L. Essential Oil—A Valuable Source of β-Elemene for Antimicrobial Testing." Molecules 23, no. 2: 256.
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