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Molecules 2018, 23(2), 256; doi:10.3390/molecules23020256

Nigella damascena L. Essential Oil—A Valuable Source of β-Elemene for Antimicrobial Testing

1
Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Chodzki 1 Street, 20-093 Lublin, Poland
2
Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1 Street, 20-093 Lublin, Poland
*
Authors to whom correspondence should be addressed.
Received: 13 December 2017 / Revised: 24 January 2018 / Accepted: 26 January 2018 / Published: 28 January 2018
(This article belongs to the Special Issue Diversity of Terpenoids)
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Abstract

The most commonly used plant source of β-elemene is Curcuma wenyujin Y. H. Chen & C. Ling (syn. of Curcuma aromatic Salisb.) with its content in supercritical CO2 extract up to 27.83%. However, the other rich source of this compound is Nigella damascena L. essential oil, in which β-elemene accounts for 47%. In this work, the effective protocol for preparative isolation of β-elemene from a new source—N. damascena essential oil—using high performance counter-current chromatography HPCCC was elaborated. Furthermore, since sesquiterpens are known as potent antimicrobials, the need for finding new agents designed to combat multi-drug resistant strains was addressed and the purified target compound and the essential oil were tested for its activity against a panel of Gram-positive and Gram-negative bacteria, fungi, and mycobacterial strains. The application of the mixture of petroleum ether, acetonitrile, and acetone in the ratio 2:1.5:0.5 (v/v) in the reversed phase mode yielded β-elemene with high purity in 70 min. The results obtained for antimicrobial assay clearly indicated that N. damascena essential oil and isolated β-elemene exert action against Mycobacterium tuberculosis strain H37Ra. View Full-Text
Keywords: GC-MS; sesquiterpenoids; ranunculaceae; essential oil; mycobacteria; tuberculosis; countercurrent separation; MIC GC-MS; sesquiterpenoids; ranunculaceae; essential oil; mycobacteria; tuberculosis; countercurrent separation; MIC
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Sieniawska, E.; Sawicki, R.; Golus, J.; Swatko-Ossor, M.; Ginalska, G.; Skalicka-Wozniak, K. Nigella damascena L. Essential Oil—A Valuable Source of β-Elemene for Antimicrobial Testing. Molecules 2018, 23, 256.

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