Pichia cactophila and Kluyveromyces lactis are Highly Efficient Microbial Cell Factories of Natural Amino Acid-Derived Aroma Compounds
AbstractThe pivotal role of non-conventional yeast (NCY) species in formation of valuable aroma compounds in various food commodities is widely acknowledged. This fact inspires endeavors aiming at exploitation of food-derived NCYs as biocatalysts in natural aromas production. In this study, we isolated, characterized and evaluated aroma-producing capacity of two NCY representatives—Pichia cactophila 7.20 and Klyuveromyces lactis 6.10 strains. The strains were isolated from food-related habitats—goat-milk regional cheese and Swiss-type ripening cheese, respectively. Aroma profiles generated by the two strains cultured in a general rich medium were analyzed through solvent extraction and GC-MS analysis of the compounds retained in the culture media. Finally, the strains were tested in bioconversion cultures with branched chain- or aromatic amino acids as the sole nitrogen source, to assess capability of the strains towards formation of amino acid-derived aromas. The results showed extraordinary capacity of both strains for production of 2-phenylethanol (at more than 3 g/L) and isoamyl alcohol (approx. 1.5 g/L). A distinctive trait of 2-phenylethyl acetate synthesis at high concentrations (0.64 g/L) was revealed for P. cactophila 7.20 strain. Highly valued disulfide dimethyl as well as methionol acetate were identified amongst the aroma compounds synthesized by the strains. View Full-Text
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Celińska, E.; Bonikowski, R.; Białas, W.; Dobrowolska, A.; Słoma, B.; Borkowska, M.; Kubiak, M.; Korpys, P.; Grajek, W. Pichia cactophila and Kluyveromyces lactis are Highly Efficient Microbial Cell Factories of Natural Amino Acid-Derived Aroma Compounds. Molecules 2018, 23, 97.
Celińska E, Bonikowski R, Białas W, Dobrowolska A, Słoma B, Borkowska M, Kubiak M, Korpys P, Grajek W. Pichia cactophila and Kluyveromyces lactis are Highly Efficient Microbial Cell Factories of Natural Amino Acid-Derived Aroma Compounds. Molecules. 2018; 23(1):97.Chicago/Turabian Style
Celińska, Ewelina; Bonikowski, Radosław; Białas, Wojciech; Dobrowolska, Anna; Słoma, Barbara; Borkowska, Monika; Kubiak, Monika; Korpys, Paulina; Grajek, Włodzimierz. 2018. "Pichia cactophila and Kluyveromyces lactis are Highly Efficient Microbial Cell Factories of Natural Amino Acid-Derived Aroma Compounds." Molecules 23, no. 1: 97.
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