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Molecules 2018, 23(1), 236; https://doi.org/10.3390/molecules23010236

Biochemical Analysis of the Role of Leucine-Rich Repeat Receptor-Like Kinases and the Carboxy-Terminus of Receptor Kinases in Regulating Kinase Activity in Arabidopsis thaliana and Brassica oleracea

1
Department of Biological Sciences, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon 34134, Korea
2
Vegetable Research Division, National Institute of Horticultural and Herbal Science, RDA, Wanju 55365, Korea
3
Department of Horticulture, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 11 December 2017 / Revised: 8 January 2018 / Accepted: 15 January 2018 / Published: 22 January 2018
(This article belongs to the Special Issue Protein Modifications and Bioconjugation)
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Abstract

Protein post-translational modification by phosphorylation is essential for the activity and stability of proteins in higher plants and underlies their responses to diverse stimuli. There are more than 300 leucine-rich repeat receptor-like kinases (LRR-RLKs), a major group of receptor-like kinases (RLKs) that plays an important role in growth, development, and biotic stress responses in higher plants. To analyze auto- and transphosphorylation patterns and kinase activities in vitro, 43 full-length complementary DNA (cDNA) sequences were cloned from genes encoding LRR-RLKs. Autophosphorylation activity was found in the cytoplasmic domains (CDs) of 18 LRR-RLKs; 13 of these LRR-RLKs with autophosphorylation activity showed transphosphorylation in Escherichia coli. BRI1-Associated Receptor Kinase (BAK1), which is critically involved in the brassinosteroid and plant innate immunity signal transduction pathways, showed strong auto- and transphosphorylation with multi-specific kinase activity within 2 h of induction of Brassica oleraceae BAK1-CD (BoBAK1-CD) in E. coli; moreover, the carboxy-terminus of LRR-RLKs regulated phosphorylation and kinase activity in Arabidopsis thaliana and vegetative crops. View Full-Text
Keywords: Arabidopsis thaliana; brassinosteroid; Brassica oleracea; leucine-rich repeat receptor-like kinases; phosphorylation Arabidopsis thaliana; brassinosteroid; Brassica oleracea; leucine-rich repeat receptor-like kinases; phosphorylation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Oh, E.-S.; Lee, Y.; Chae, W.B.; Rameneni, J.J.; Park, Y.-S.; Lim, Y.P.; Oh, M.-H. Biochemical Analysis of the Role of Leucine-Rich Repeat Receptor-Like Kinases and the Carboxy-Terminus of Receptor Kinases in Regulating Kinase Activity in Arabidopsis thaliana and Brassica oleracea. Molecules 2018, 23, 236.

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