Next Article in Journal
Development of Antimicrobial Biocomposite Films to Preserve the Quality of Bread
Previous Article in Journal
Immobilization of Staphylococcus aureus Sortase A on Chitosan Particles and Its Applications in Peptide-to-Peptide Ligation and Peptide Cyclization
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Molecules 2018, 23(1), 214; https://doi.org/10.3390/molecules23010214

Identification of Cyclic Dipeptides from Escherichia coli as New Antimicrobial Agents against Ralstonia Solanacearum

1,2,†
,
1,2,3,†
,
1,2,3,†
,
4
,
5
,
1,2
,
3
and
1,2,3,*
1
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China
2
Guangdong Innovative Research Team of Sociomicrobiology, College of Agriculture, South China Agricultural University, Guangzhou 510642, China
3
Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China
4
School of Biological and Science Technology, University of Jinan, Jinan 250022, China
5
Department of Chemistry, National University of Singapore, Science Drive 3, Singapore 117543, Singapore
These authors have contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 11 December 2017 / Revised: 15 January 2018 / Accepted: 18 January 2018 / Published: 19 January 2018
(This article belongs to the Section Natural Products Chemistry)
Full-Text   |   PDF [2743 KB, uploaded 19 January 2018]   |  

Abstract

Ralstonia solanacearum is a causative agent of bacterial wilt in many important crops throughout the world. How to control bacterial wilt caused by R. solanacearum is a major problem in agriculture. In this study, we aim to isolate the biocontrol agents that have high efficacy in the control of bacterial wilt. Three new bacterial strains with high antimicrobial activity against R. solanacearum GMI1000 were isolated and identified. Our results demonstrated that these bacteria could remarkably inhibit the disease index of host plant infected by R. solanacearum. It was indicated that strain GZ-34 (CCTCC No. M 2016353) showed an excellent protective effect to tomato under greenhouse conditions. Strain GZ-34 was characterized as Escherichia coli based on morphology, biochemistry, and 16S rRNA analysis. We identified that the main antimicrobial compounds produced by E. coli GZ-34 were cyclo(l-Pro-d-Ile) and cyclo(l-Pro-l-Phe) using electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) analysis. The two active compounds also interfered with the expression levels of some pathogenicity-contributors of R. solanacearum. Furthermore, cyclo(l-Pro-l-Phe) effectively inhibited spore formation of Magnaporthe grisea, which is a vital pathogenesis process of the fungal pathogen, suggesting cyclic dipeptides from E. coli are promising potential antimicrobial agents with broad-spectrum activity to kill pathogens or interfere with their pathogenesis. View Full-Text
Keywords: bacterial wilt; Ralstonia solanacearum; biocontrol agent; antimicrobial activity; cyclic dipeptides bacterial wilt; Ralstonia solanacearum; biocontrol agent; antimicrobial activity; cyclic dipeptides
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Song, S.; Fu, S.; Sun, X.; Li, P.; Wu, J.; Dong, T.; He, F.; Deng, Y. Identification of Cyclic Dipeptides from Escherichia coli as New Antimicrobial Agents against Ralstonia Solanacearum. Molecules 2018, 23, 214.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top