Constituents of the Fruits of Citrus medica L. var. sarcodactylis and the Effect of 6,7-Dimethoxy-coumarin on Superoxide Anion Formation and Elastase Release
Abstract
:1. Introduction
2. Results and Discussion
2.1. Purification and Characterization
2.2. Structural Elucidation of Compound 1
2.3. The Inhibitory Activity of Superoxide Anion Generation and Elastase Release
3. Materials and Methods
3.1. General Information
3.2. Materials
3.3. Extraction and Isolation
3.4. Bioactivity Examination
3.4.1. Preparation of Human Neutrophils
3.4.2. Inhibition of Superoxide Anion Generation
3.4.3. Inhibition of Elastase Release
3.4.4. Statistical Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the purified compounds are available from the authors. |
Position | δH (mult., J in Hz) | δC |
---|---|---|
1 | 168.2 | |
2 | 5.79 (1H, s) | 118.3 |
3 | 152.0 | |
4 | 8.00 (1H, d, 15.7) | 131.7 |
5 | 6.55 (1H, d, 15.7) | 135.7 |
1′ | 83.3 | |
2′ | 87.8 | |
3′ | 2.03 (1H, dd, 13.7, 7.0) 1.73 (1H, dd, 13.7, 10.4) | 46.0 |
4′ | 4.12 (1H, m) | 66.0 |
5′ | 1.86 (1H, dd, 13.5, 6.9) 1.68 (1H, dd, 13.5, 13.5) | 44.6 |
6′ | 49.2 | |
7′ | 1.16 (3H, s) | 19.6 |
8′ | 0.94 (3H, s) | 16.4 |
9′ | 3.82 (1H, d, 7.5) 3.72 (1H, d, 7.5) | 77.3 |
CH3-3 | 2.10 (3H, s,) | 21.2 |
OCH3 | 3.70 (3H, s) | 51.6 |
Compound | IC50 (μM) a | |
---|---|---|
Superoxide Anion Generation | Elastase Release | |
5 | 3.8 ± 1.4 *** | >10 |
LY294002 b | 0.4 ± 0.02 *** | 1.5 ± 0.3 *** |
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Chan, Y.-Y.; Hwang, T.-L.; Kuo, P.-C.; Hung, H.-Y.; Wu, T.-S. Constituents of the Fruits of Citrus medica L. var. sarcodactylis and the Effect of 6,7-Dimethoxy-coumarin on Superoxide Anion Formation and Elastase Release. Molecules 2017, 22, 1454. https://doi.org/10.3390/molecules22091454
Chan Y-Y, Hwang T-L, Kuo P-C, Hung H-Y, Wu T-S. Constituents of the Fruits of Citrus medica L. var. sarcodactylis and the Effect of 6,7-Dimethoxy-coumarin on Superoxide Anion Formation and Elastase Release. Molecules. 2017; 22(9):1454. https://doi.org/10.3390/molecules22091454
Chicago/Turabian StyleChan, Yu-Yi, Tsong-Long Hwang, Ping-Chung Kuo, Hsin-Yi Hung, and Tian-Shung Wu. 2017. "Constituents of the Fruits of Citrus medica L. var. sarcodactylis and the Effect of 6,7-Dimethoxy-coumarin on Superoxide Anion Formation and Elastase Release" Molecules 22, no. 9: 1454. https://doi.org/10.3390/molecules22091454