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Molecules 2017, 22(9), 1427; doi:10.3390/molecules22091427

Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position

1
Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
2
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA
3
Theoretical Biology and Biophysics Group, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
*
Author to whom correspondence should be addressed.
Received: 25 July 2017 / Revised: 15 August 2017 / Accepted: 22 August 2017 / Published: 29 August 2017
(This article belongs to the Special Issue Nucleoside and Nucleotide Analogues)
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Abstract

Accurate translation of the genetic code depends on mRNA:tRNA codon:anticodon base pairing. Here we exploit an emissive, isosteric adenosine surrogate that allows direct measurement of the kinetics of codon:anticodon University of California base formation during protein synthesis. Our results suggest that codon:anticodon base pairing is subject to tighter constraints at the middle position than at the 5′- and 3′-positions, and further suggest a sequential mechanism of formation of the three base pairs in the codon:anticodon helix. View Full-Text
Keywords: fluorescent mRNA; mRNA:tRNA base pairing; kinetic mechanism; steric constraint; atomic mutagenesis fluorescent mRNA; mRNA:tRNA base pairing; kinetic mechanism; steric constraint; atomic mutagenesis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Liu, W.; Shin, D.; Ng, M.; Sanbonmatsu, K.Y.; Tor, Y.; Cooperman, B.S. Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position. Molecules 2017, 22, 1427.

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