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Molecules 2017, 22(9), 1388; doi:10.3390/molecules22091388

Determination of Coenzyme A and Acetyl-Coenzyme A in Biological Samples Using HPLC with UV Detection

1
Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10065, USA
2
Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
3
Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia
4
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
*
Authors to whom correspondence should be addressed.
Received: 19 July 2017 / Accepted: 12 August 2017 / Published: 23 August 2017
(This article belongs to the Section Metabolites)
View Full-Text   |   Download PDF [1327 KB, uploaded 23 August 2017]   |  

Abstract

Coenzyme A (CoA) and acetyl-coenzyme A (acetyl-CoA) play essential roles in cell energy metabolism. Dysregulation of the biosynthesis and functioning of both compounds may contribute to various pathological conditions. We describe here a simple and sensitive HPLC-UV based method for simultaneous determination of CoA and acetyl-CoA in a variety of biological samples, including cells in culture, mouse cortex, and rat plasma, liver, kidney, and brain tissues. The limits of detection for CoA and acetyl-CoA are >10-fold lower than those obtained by previously described HPLC procedures, with coefficients of variation <1% for standard solutions, and 1–3% for deproteinized biological samples. Recovery is 95–97% for liver extracts spiked with Co-A and acetyl-CoA. Many factors may influence the tissue concentrations of CoA and acetyl-CoA (e.g., age, fed, or fasted state). Nevertheless, the values obtained by the present HPLC method for the concentration of CoA and acetyl-CoA in selected rodent tissues are in reasonable agreement with literature values. The concentrations of CoA and acetyl-CoA were found to be very low in rat plasma, but easily measurable by the present HPLC method. The method should be useful for studying cellular energy metabolism under normal and pathological conditions, and during targeted drug therapy treatment. View Full-Text
Keywords: Acetyl-coenzyme A; coenzyme A; high performance liquid chromatography; UV detection Acetyl-coenzyme A; coenzyme A; high performance liquid chromatography; UV detection
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Shurubor, Y.I.; D’Aurelio, M.; Clark-Matott, J.; Isakova, E.P.; Deryabina, Y.I.; Beal, M.F.; Cooper, A.J.L.; Krasnikov, B.F. Determination of Coenzyme A and Acetyl-Coenzyme A in Biological Samples Using HPLC with UV Detection. Molecules 2017, 22, 1388.

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