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Molecules 2017, 22(7), 1054; doi:10.3390/molecules22071054

Rational Design of Cyclic Antimicrobial Peptides Based on BPC194 and BPC198

1
LIPPSO, Departament de Química, University of Girona, Maria Aurèlia Capmany 69, 17003 Girona, Spain
2
Institut de Química Computacional i Catàlisi i Departament de Química, University of Girona, Maria Aurèlia Capmany 69, 17003 Girona, Spain
3
Laboratory of Plant Pathology, Institute of Food and Agricultural Technology-CIDSAV-XaRTA, University of Girona, Maria Aurèlia Capmany 61, 17003 Girona, Spain
*
Authors to whom correspondence should be addressed.
Received: 29 May 2017 / Revised: 20 June 2017 / Accepted: 22 June 2017 / Published: 24 June 2017
(This article belongs to the Special Issue Peptide-Based Drugs and Drug Delivery Systems)
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Abstract

A strategy for the design of antimicrobial cyclic peptides derived from the lead compounds c(KKLKKFKKLQ) (BPC194) and c(KLKKKFKKLQ) (BPC198) is reported. First, the secondary β-structure of BPC194 and BPC198 was analyzed by carrying out molecular dynamics (MD) simulations. Then, based on the sequence pattern and the β-structure of BPC194 or BPC198, fifteen analogues were designed and synthesized on solid-phase. The best peptides (BPC490, BPC918, and BPC924) showed minimum inhibitory concentration (MIC) values <6.2 μM against Pseudomonas syringae pv. syringae and Xanthomonas axonopodis pv. vesicatoria, and an MIC value of 12.5 to 25 μM against Erwinia amylovora, being as active as BPC194 and BPC198. Interestingly, these three analogues followed the structural pattern defined from the MD simulations of the parent peptides. Thus, BPC490 maintained the parallel alignment of the hydrophilic pairs K1–K8, K2–K7, and K4–K5, whereas BPC918 and BPC924 included the two hydrophilic interactions K3–Q10 and K5–K8. In short, MD simulations have proved to be very useful for ascertaining the structural features of cyclic peptides that are crucial for their biological activity. Such approaches could be further employed for the development of new antibacterial cyclic peptides. View Full-Text
Keywords: antimicrobial peptides; rational design; molecular dynamics; cyclopeptides; plant pathogens; secondary structure antimicrobial peptides; rational design; molecular dynamics; cyclopeptides; plant pathogens; secondary structure
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Cirac, A.D.; Torné, M.; Badosa, E.; Montesinos, E.; Salvador, P.; Feliu, L.; Planas, M. Rational Design of Cyclic Antimicrobial Peptides Based on BPC194 and BPC198. Molecules 2017, 22, 1054.

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