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Molecules 2017, 22(6), 927; doi:10.3390/molecules22060927

Simultaneous Quantification of Multiple Representative Components in the Xian-Ling-Gu-Bao Capsule by Ultra-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry

1,2,†,* , 1,2,3,†
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1
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1,2,* , 4
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4
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1,2,3
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1
College of Pharmacy, Jinan University, Guangzhou 510632, China
2
Guangdong Provincial Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China
3
G Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan University, Guangzhou 510632, China
4
Musculoskeletal Research Laboratory, Department of Orthopedics and Traumatology, the Chinese University of Hong Kong, Satin, N.T. Hong Kong SAR, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 19 April 2017 / Revised: 25 May 2017 / Accepted: 1 June 2017 / Published: 2 June 2017
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Abstract

Xian-Ling-Gu-Bao capsule (XLGB), a famous traditional Chinese medicine prescription, is extensively used for the treatment of osteoporosis in China. However, few studies on the holistic quality control of XLGB have been reported. In this study, a reliable method using 18 representative components in XLGB was successfully established and applied to evaluate 34 batches of XLGB samples by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS). The choice of quantitative markers mostly followed four principles, i.e., absorbed components in plasma, bioactive compounds with in vitro anti-osteoporosis activity, those derived from multiple individual medicinal herbs in XLGB with multiple representative structure types, and quantitative chemical markers in the Chinese Pharmacopoeia. The results showed chemical consistency was good except for individual batches. Multivariate statistical analysis indicated that asperosaponin VI from Radix Dipsaci, epimedin C, magnoflorine, and icariin from Herba Epimedii as well as timosaponin BII from Rhizoma Anemarrhenae varied significantly in multiple samples, which hinted an assay for these four components should be completed during all of the manufacturing processes. Taken together, this study provided a feasible method for holistic quality control of XLGB by multiple chemical markers, which could play a vital role in guaranteeing the safety, effectiveness, and controllability of administering the capsules as a medication in clinics. View Full-Text
Keywords: Xian-Ling-Gu-Bao capsule; representative components; quantification; quality control; UPLC/Q-TOF-MS Xian-Ling-Gu-Bao capsule; representative components; quantification; quality control; UPLC/Q-TOF-MS
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Yao, Z.-H.; Qin, Z.-F.; Cheng, H.; Wu, X.-M.; Dai, Y.; Wang, X.-L.; Qin, L.; Ye, W.-C.; Yao, X.-S. Simultaneous Quantification of Multiple Representative Components in the Xian-Ling-Gu-Bao Capsule by Ultra-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry. Molecules 2017, 22, 927.

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