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Molecules 2017, 22(6), 901; doi:10.3390/molecules22060901

Expression of Genes Related to Phenylpropanoid Biosynthesis in Different Organs of Ixeris dentata var. albiflora

1
Department of Herb Crop Resources, National Institute of Horticultural & Herbal Science, RDA, Eumseong-gun 27709, Korea
2
Department of Biology, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea
3
Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 28 February 2017 / Revised: 26 May 2017 / Accepted: 26 May 2017 / Published: 30 May 2017
(This article belongs to the Section Natural Products)
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Abstract

Members of the genus Ixeris have long been used in traditional medicines as stomachics, sedatives, and diuretics. Phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate: coenzyme-A (CoA) ligase (4CL), chalcone synthase (CHS), and dihydroflavonol 4-reductase (DFR) are important enzymes in the phenylpropanoid pathway. In this study, we analyzed seven genes from Ixeris dentata var. albiflora that are involved in phenylpropanoid biosynthesis, using an Illumina/Solexa HiSeq 2000 platform. The amino acid sequence alignments for IdPALs, IdC4H, Id4CLs, IdCHS, and IdDFR showed high identity to sequences from other plants. We also investigated transcript levels using quantitative real-time PCR, and analyzed the accumulation of phenylpropanoids in different organs of I. dentata var. albiflora using high-performance liquid chromatography. The transcript levels of IdC4H, Id4CL1, IdCHS, and IdDFR were highest in the leaf. The catechin, chlorogenic acid, ferulic acid, and quercetin contents were also highest in the leaf. We suggest that expression of IdC4H, Id4CL1, IdCHS, and IdDFR is associated with the accumulation of phenylpropanoids. Our results may provide baseline information for elucidating the mechanism of phenylpropanoid biosynthesis in different organs of I. dentata var. albiflora. View Full-Text
Keywords: Ixeris dentata var. albiflora; phenylpropanoid; transcript level Ixeris dentata var. albiflora; phenylpropanoid; transcript level
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Lee, S.-H.; Park, Y.-J.; Park, S.U.; Lee, S.-W.; Kim, S.-C.; Jung, C.-S.; Jang, J.-K.; Hur, Y.; Kim, Y.B. Expression of Genes Related to Phenylpropanoid Biosynthesis in Different Organs of Ixeris dentata var. albiflora. Molecules 2017, 22, 901.

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