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Molecules 2017, 22(4), 624; doi:10.3390/molecules22040624

LC-ESI-MS/MS Identification of Biologically Active Phenolic Compounds in Mistletoe Berry Extracts from Different Host Trees

1
Department of Pharmaceutical Botany, Medical University of Lublin, Chodźki 1 Str., 20-093 Lublin, Poland
2
Department of Biochemistry and Food Chemistry, University of Life Sciences, Skromna 8 Str., 20-704 Lublin, Poland
3
Department of Medical Biology, Institute of Rural Health in Lublin, Jaczewskiego 2, 20-090 Lublin, Poland
4
Department of Virology and Immunology, Maria Curie Skłodowska University, Akademicka 19, 20-033 Lublin, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Isabel C.F.R. Ferreira
Received: 22 March 2017 / Revised: 5 April 2017 / Accepted: 6 April 2017 / Published: 12 April 2017
(This article belongs to the Collection Bioactive Compounds)
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Abstract

A new, rapid, sensitive and selective liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method was developed to determine the content of flavonoid aglycones and phenolic acids in mistletoe berries (Viscum album L.) harvested from six different Polish host trees. Additionally, the total phenolic content (TPC) and total flavonoid content (TFC) as well as an antioxidant and antiproliferative activity were evaluated for the first time. The plant material was selectively extracted using ultrasound assisted maceration with methanol/water (8:2) solution. The obtained TPC and TFC results varied from 7.146 to 9.345 mg GA g1 and from 1.888 to 2.888 mg Q g−1 of dry extracts, respectively. The LC-ESI-MS/MS analysis demonstrated the highest content of phenolic acids in mistletoe berries from Populus nigra ‘Italica’ L. and flavonoid aglycones in mistletoe berries from Tilia cordata Mill. (354.45 µg and 5.955 µg per g dry extract, respectively). The moderate antioxidant activity of investigated extracts was obtained. The studies revealed that the examined extracts decreased the proliferation of human colon adenocarcinoma cells line LS180 in a dose-dependent manner without cytotoxicity in the human colon epithelial cell line CCD 841 CoTr. Moreover, the obtained results suggest considerable impact of polyphenols on the anticancer activity of these extracts. View Full-Text
Keywords: antioxidant activity; antiproliferative activity; cytotoxicity; LC-MS/MS (mass spectrometry); phenolic compound; Viscum album L. antioxidant activity; antiproliferative activity; cytotoxicity; LC-MS/MS (mass spectrometry); phenolic compound; Viscum album L.
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Pietrzak, W.; Nowak, R.; Gawlik-Dziki, U.; Lemieszek, M.K.; Rzeski, W. LC-ESI-MS/MS Identification of Biologically Active Phenolic Compounds in Mistletoe Berry Extracts from Different Host Trees. Molecules 2017, 22, 624.

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