Next Article in Journal
Bergamot Essential Oil Attenuates Anxiety-Like Behaviour in Rats
Next Article in Special Issue
Heparin, Heparan Sulphate and the TGF-β Cytokine Superfamily
Previous Article in Journal
Anti-Inflammatory, Antioxidant and Crystallographic Studies of N-Palmitoyl-ethanol Amine (PEA) Derivatives
Previous Article in Special Issue
Non-Anticoagulant Heparins Are Hepcidin Antagonists for the Treatment of Anemia
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessReview
Molecules 2017, 22(4), 617; doi:10.3390/molecules22040617

Functional Assays in the Diagnosis of Heparin-Induced Thrombocytopenia: A Review

1
Department of Pharmacy, Namur Thrombosis and Hemostasis Center (NTHC), Namur Research Institute for LIfe Sciences (NARILIS), University of Namur, Namur 5000, Belgium
2
CHU UCL Namur, Namur Thrombosis and Hemostasis Center (NTHC), Hematology Laboratory, Université catholique de Louvain, Yvoir 5530, Belgium
*
Author to whom correspondence should be addressed.
Academic Editors: Giangiacomo Torri and Jawed Fareed
Received: 15 March 2017 / Revised: 5 April 2017 / Accepted: 8 April 2017 / Published: 11 April 2017
View Full-Text   |   Download PDF [724 KB, uploaded 11 April 2017]   |  

Abstract

A rapid and accurate diagnosis in patients with suspected heparin-induced thrombocytopenia (HIT) is essential for patient management but remains challenging. Current HIT diagnosis ideally relies on a combination of clinical information, immunoassay and functional assay results. Platelet activation assays or functional assays detect HIT antibodies that are more clinically significant. Several functional assays have been developed and evaluated in the literature. They differ in the activation endpoint studied; the technique or technology used; the platelet donor selection; the platelet suspension (washed platelets, platelet rich plasma or whole blood); the patient sample (serum or plasma); and the heparin used (type and concentrations). Inconsistencies in controls performed and associated results interpretation are common. Thresholds and performances are determined differently among papers. Functional assays suffer from interlaboratory variability. This lack of standardization limits the evaluation and the accessibility of functional assays in laboratories. In the present article, we review all the current activation endpoints, techniques and methodologies of functional assays developed for HIT diagnosis. View Full-Text
Keywords: heparin-induced thrombocytopenia; diagnosis; functional assay; platelets heparin-induced thrombocytopenia; diagnosis; functional assay; platelets
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Minet, V.; Dogné, J.-M.; Mullier, F. Functional Assays in the Diagnosis of Heparin-Induced Thrombocytopenia: A Review. Molecules 2017, 22, 617.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top