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Molecules 2017, 22(4), 530; doi:10.3390/molecules22040530

Hydrogen Sulphide Production in Healthy and Ulcerated Gastric Mucosa of Rats

1
Chair of Medical Biochemistry, Jagiellonian University Medical College, Krakow, 7 Kopernika St., 31-034 Cracow, Poland
2
Department of Physiology, Jagiellonian University Medical College, 31-530 Krakow, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 27 January 2017 / Revised: 13 March 2017 / Accepted: 22 March 2017 / Published: 27 March 2017
(This article belongs to the Special Issue Sulfur Atom: Element for Adaptation to an Oxidative Environment 2016)
View Full-Text   |   Download PDF [2190 KB, uploaded 28 March 2017]   |  

Abstract

Hydrogen sulphide (H2S) is produced endogenously via two enzymes dependent on pyridoxal phosphate (PLP): cystathionine beta-synthase (CBS, EC 4.2.1.22), cystathionase γ-liase (CTH, EC 4.4.1.1), and a third, 3-mercaptopyruvate sulfurtransferase (MPST, EC 2.8.1.2). H2S strengthens the defence mechanisms of the gastric mucosal barrier, and plays an important role in gastroprotection, including the increased resistance to damage caused by various irritants and non-steroidal anti-inflammatory drugs. The study was conducted to determine the role of H2S in ulcerated gastric mucosa of rats caused by immobilization in cold water (WRS). The activity and expression of γ-cystathionase, cystathionine β-synthase, 3-mercaptopyruvate sulfurtransferase, and rhodanese was compared with healthy mucosa, together with H2S generation, and cysteine, glutathione, and cystathionine levels. The results showed that the defence mechanism against stress is associated with stimulation of the production of H2S in the tissue and confirmed the observed advantageous effect of H2S on healing of gastric ulcers. In case of animals pretreated with exogenous sources of H2S and NaHS, and some changes observed in the ulcerated gastric mucosa tend to return to values found in the healthy tissue, a finding that is in accordance with the previously determined gastroprotective properties of H2S. The results presented in this paper point to the possible role of rhodanese in H2S production in the gastric mucosa of rats, together with the earlier mentioned three enzymes, which are all active in this tissue. View Full-Text
Keywords: hydrogen sulphide; gastric mucosa; NaHS; cystathionine-β-synthase; 3-mercaptopyruvate sulfurtransferase; rhodanese; γ-cystathionase hydrogen sulphide; gastric mucosa; NaHS; cystathionine-β-synthase; 3-mercaptopyruvate sulfurtransferase; rhodanese; γ-cystathionase
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Bronowicka-Adamska, P.; Wróbel, M.; Magierowski, M.; Magierowska, K.; Kwiecień, S.; Brzozowski, T. Hydrogen Sulphide Production in Healthy and Ulcerated Gastric Mucosa of Rats. Molecules 2017, 22, 530.

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