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Molecules 2017, 22(11), 2030; doi:10.3390/molecules22112030

The Rosiglitazone-Like Effects of Vitexilactone, a Constituent from Vitex trifolia L. in 3T3-L1 Preadipocytes

1
College of Science and Technology, Nihon University, 1-5-1 Kandasurugadai, Chiyoda, Tokyo 101-0062, Japan
2
Department of Biomedical Information Engineering, Graduate School of Medical Science, Yamagata University, 2-2-2 Iidanishi, Yamagata 990-9585, Japan
3
School of Medical Technology, Faculty of Health Science, Gunma Paz University, 1-7-1 Tonyamachi, Takasaki, Gunma 370-0006, Japan
4
Faculty of Arts and Sciences, Showa University, 4562 Kamiyoshida, Fujiyoshida, Yamanashi 403-0005, Japan
5
Botany Department, Pathein University, Main Rd., Pathein, Myanmar
6
School of Medicine, Nihon University, 30-1 Oyaguchi-kamicho, Itabashi, Tokyo 173-8610, Japan
*
Author to whom correspondence should be addressed.
Received: 18 October 2017 / Revised: 8 November 2017 / Accepted: 17 November 2017 / Published: 22 November 2017
(This article belongs to the Collection Bioactive Compounds)
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Abstract

The increased number of patients with type 2 diabetes (T2D) has become a worldwide problem, and insulin sensitizers such as thiazolidinediones (TZDs) are used as therapeutic agents. We found that extracts of Vitex trifolia L. (V. trifolia), a medicinal plant from Myanmar, induced adipogenesis similar to rosiglitazone (ROS), which is a TZD, in 3T3-L1 preadipocytes. In the present study, we attempted to isolate from V. trifolia those compounds that showed ROS-like effects. Among the extracts of hexane, ethyl acetate, and methanol obtained from V. trifolia, the ethyl acetate extract with the strongest ROS-like effects was purified by various chromatographic methods to obtain three known compounds: vitexilactone (1), vitexicarpin (2) and oleanolic acid (3). Among the isolated compounds, the ROS-like action of 1 was the strongest. The effects of 1 on 3T3-L1 cells during adipogenesis were compared with those of ROS. Both 1 and ROS increased lipid accumulation, the expression of adiponectin and GLUT4 in the cell membrane and decreased both the size of adipocytes and the phosphorylation of IRS-1, ERK1/2 and JNK in 3T3-L1 cells. In contrast, unlike ROS, the induction of proteins involved in lipogenesis was partial. ROS-like effects of 1 in 3T3-L1 cells were suppressed by the addition of bisphenol A diglycidyl ether (BADGE), one of a peroxisome proliferator-activated receptor γ (PPARγ) antagonists, suggesting that the action of 1 on adipocytes is mediated by PPARγ. From the results of the present study, it can be concluded that 1 is a novel insulin sensitizer candidate. View Full-Text
Keywords: Vitex trifolia L.; adipogenesis; lipolysis; lipogenesis; rosiglitazone; vitexilactone Vitex trifolia L.; adipogenesis; lipolysis; lipogenesis; rosiglitazone; vitexilactone
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MDPI and ACS Style

Nishina, A.; Itagaki, M.; Sato, D.; Kimura, H.; Hirai, Y.; Phay, N.; Makishima, M. The Rosiglitazone-Like Effects of Vitexilactone, a Constituent from Vitex trifolia L. in 3T3-L1 Preadipocytes. Molecules 2017, 22, 2030.

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