Next Article in Journal
Four New Glycosides from the Rhizoma of Anemarrhena asphodeloides
Previous Article in Journal
The Complete Chloroplast Genome Sequences of Aconitum pseudolaeve and Aconitum longecassidatum, and Development of Molecular Markers for Distinguishing Species in the Aconitum Subgenus Lycoctonum
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Molecules 2017, 22(11), 2021; doi:10.3390/molecules22112021

Transformation of a Thermostable G-Quadruplex Structure into DNA Duplex Driven by Reverse Gyrase

1
Key Lab of Forest Genetics and Biotechnology, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China
2
Jiangsu Key Laboratory for Biofunctional Molecules, College of Life Science and Chemistry, Jiangsu Second Normal University, Nanjing 210037, China
*
Authors to whom correspondence should be addressed.
Received: 8 November 2017 / Revised: 14 November 2017 / Accepted: 17 November 2017 / Published: 22 November 2017
View Full-Text   |   Download PDF [1607 KB, uploaded 22 November 2017]   |  

Abstract

Reverse gyrase is a topoisomerase that can introduce positive supercoils to its substrate DNA. It is demonstrated in our studies that a highly thermal stable G-quadruplex structure in a mini-plasmid DNA was transformed into its duplex conformation after a treatment with reverse gyrase. The structural difference of the topoisomers were verified and analyzed by gel electrophoresis, atomic force microscopy examination, and endonuclease digestion assays. All evidence suggested that the overwinding structure of positive supercoil could provide a driven force to disintegrate G-quadruplex and reform duplex. The results of our studies could suggest that hyperthermophiles might use reverse gyrase to manipulate the disintegration of non-B DNA structures and safekeep their genomic information. View Full-Text
Keywords: G-quadruplex; DNA supercoiling; topoisomer; topoisomerase; Atomic Force Microscope examination G-quadruplex; DNA supercoiling; topoisomer; topoisomerase; Atomic Force Microscope examination
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Li, D.; Wang, Q.; Liu, Y.; Liu, K.; Zhuge, Q.; Lv, B. Transformation of a Thermostable G-Quadruplex Structure into DNA Duplex Driven by Reverse Gyrase. Molecules 2017, 22, 2021.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top