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Molecules 2017, 22(11), 1992; doi:10.3390/molecules22111992

A New UPLC-MS/MS Method for the Characterization and Discrimination of Polysaccharides from Genus Ephedra Based on Enzymatic Digestions

Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China
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Received: 29 October 2017 / Accepted: 15 November 2017 / Published: 17 November 2017
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Abstract

Ephedra sinica polysaccharides have been reported to possess important activities, so quality evaluation of polysaccharides from the genus Ephedra is urgent. In this study, enzymatic digestions were performed to establish multiple saccharide fingerprints by ultra-performance liquid chromatography with electrospray ionization triple quadrupole linear ion trap mass spectrometry (UPLC-ESI-TQ-MS/MS) based on a multiple-reaction monitoring in negative mode. Under optimum UPLC-ESI--TQ-MS/MS conditions, excellent separation and quantification of 21 constituents were achieved within 20 min on a solid core column with a 1.6 μm particle using pre-column derivatization with a PMP reagent. This method, coupled with enzymatic digestions and principal component analysis, has been successfully applied to characterize and discriminate Ephedra polysaccharides attributed to different species and plant parts. The results suggest that the proposed analytical strategy could achieve a quality evaluation of plant polysaccharides from traditional Chinese medicines. View Full-Text
Keywords: UPLC-MS/MS; polysaccharides; genus Ephedra; enzymatic digestion UPLC-MS/MS; polysaccharides; genus Ephedra; enzymatic digestion
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Xia, Y.-G.; Wang, T.-L.; Sun, L.-M.; Liang, J.; Yang, B.-Y.; Kuang, H.-X. A New UPLC-MS/MS Method for the Characterization and Discrimination of Polysaccharides from Genus Ephedra Based on Enzymatic Digestions. Molecules 2017, 22, 1992.

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