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Molecules 2017, 22(11), 1877; doi:10.3390/molecules22111877

Optimization of the Extraction Conditions for Buddleja officinalis Maxim. Using Response Surface Methodology and Exploration of the Optimum Harvest Time

Department of Resources Science of Traditional Chinese Medicines, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China
These authors contributed equally to this work.
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Received: 30 September 2017 / Accepted: 29 October 2017 / Published: 1 November 2017
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Abstract

The Box-Behnken design was used to evaluate the effects of the methanol concentration (60–100%), liquid to solid ratio (20:1 to 40:1 mL/g) and extraction time (20–40 min) on the yield of 11 constituents from Buddleja officinalis Maxim using ultrasound-assisted extraction. The Derringer’s desirability function approach showed that the modified optimum extraction conditions were: 76% methanol concentration, 33 min extraction time and a 34:1 mL/g solvent to solid ratio. Under these conditions, the experimentally measured yields of the compounds were in good agreement with the predicted values. An accurate and sensitive method was also established using high-performance liquid chromatography with diode-array detection for the simultaneous determination of the 11 compounds in Buddleja officinalis. The newly developed method was used to determine the amounts of bioactive components in Buddleja officinalis during four different growth stages. According to these results, we recommend that the full blossom stage is the best time for harvesting this plant to obtain the highest yield of crude materials. View Full-Text
Keywords: Buddleja officinalis Maxim; multiple responses optimization; optimum harvest time; response surface methodology Buddleja officinalis Maxim; multiple responses optimization; optimum harvest time; response surface methodology
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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    Description: Table S1 ANOVA results of eleven responses Table S2 The content of 11 bioactive compounds in B. officinalis at different growth stages (Mean±SD, mg/g, n=3)

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Xie, G.; Li, R.; Han, Y.; Zhu, Y.; Wu, G.; Qin, M. Optimization of the Extraction Conditions for Buddleja officinalis Maxim. Using Response Surface Methodology and Exploration of the Optimum Harvest Time. Molecules 2017, 22, 1877.

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