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Molecules 2017, 22(11), 1866; doi:10.3390/molecules22111866

Purification, Characterization, and Antioxidant Activity of Polysaccharides Isolated from Cortex Periplocae

College of Tobacco Science/National Tobacco Cultivation & Physiology & Biochemistry Research Center, Henan Agricultural University, Zhengzhou 450002, China
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Received: 13 October 2017 / Revised: 27 October 2017 / Accepted: 30 October 2017 / Published: 31 October 2017
(This article belongs to the Special Issue Advances in Natural Polysaccharides Research)
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Abstract

In this study, crude Cortex Periplocae polysaccharides (CCPPs) were extracted with water. CCPPs were decolored with AB-8 resin and deproteinated using papain-Sevage methods. Then, they were further purified and separated through DEAE-52 anion exchange chromatography and Sephadex G-100 gel filtration chromatography, respectively. Three main fractions—CPP1, CPP2, and CPP3, (CPPs)—were obtained. The average molecular weights, monosaccharide analysis, surface morphology, and chemical compositions of the CPPs were investigated by high-performance gel permeation chromatography (HPGPC), gas chromatography-mass spectrometry (GC/MS), UV-vis spectroscopy, Fourier transform infrared (FT-IR) spectrum, and nuclear magnetic resonance (NMR). In addition, the antioxidant activities of these three polysaccharides were investigated. The results indicated that all of the CPPs were composed of rhamnose, arabinose, mannose, glucose, and galactose. These three polysaccharides exhibited antioxidant activities in four assays including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2′-azino-bis(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS) radical, reducing power, and total antioxidant activity in vitro. The data indicated that these three polysaccharides could be utilized as potential natural sources of alternative additives in the functional food, cosmetics, and pharmaceutical industries. View Full-Text
Keywords: Cortex Periplocae; polysaccharides; purification; chemical composition; antioxidant activity Cortex Periplocae; polysaccharides; purification; chemical composition; antioxidant activity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wang, X.; Zhang, Y.; Liu, Z.; Zhao, M.; Liu, P. Purification, Characterization, and Antioxidant Activity of Polysaccharides Isolated from Cortex Periplocae. Molecules 2017, 22, 1866.

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