Anti-Inflammatory Phenolic Metabolites from the Edible Fungus Phellinus baumii in LPS-Stimulated RAW264.7 Cells
Abstract
:1. Introduction
2. Results & Discussion
2.1. Bioactivity-Guided Fractionation for Anti-Inflammatory Effects
2.2. Chemical Investigation and Identification of the Active Compounds
2.3. Effects of Compounds 1–8 on NO Production
2.4. Effects of Compounds 1–3 on Proteins Associated with Inhibition of NF-κB in LPS-Stimulated RAW264.7 Mouse Macrophages
3. Materials and Methods
3.1. General Experimental Procedures
3.2. Fungus Material
3.3. Extraction, Fractionation, and Purification Methods
3.4. Cell Culture and MTT Cell Viability Assay
3.5. Measurement of Nitric Oxide Production
3.6. Western Blotting Analysis
3.7. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Lee, S.; Lee, D.; Jang, T.S.; Kang, K.S.; Nam, J.-W.; Lee, H.-J.; Kim, K.H. Anti-Inflammatory Phenolic Metabolites from the Edible Fungus Phellinus baumii in LPS-Stimulated RAW264.7 Cells. Molecules 2017, 22, 1583. https://doi.org/10.3390/molecules22101583
Lee S, Lee D, Jang TS, Kang KS, Nam J-W, Lee H-J, Kim KH. Anti-Inflammatory Phenolic Metabolites from the Edible Fungus Phellinus baumii in LPS-Stimulated RAW264.7 Cells. Molecules. 2017; 22(10):1583. https://doi.org/10.3390/molecules22101583
Chicago/Turabian StyleLee, Seulah, Dahae Lee, Tae Su Jang, Ki Sung Kang, Joo-Won Nam, Hae-Jeung Lee, and Ki Hyun Kim. 2017. "Anti-Inflammatory Phenolic Metabolites from the Edible Fungus Phellinus baumii in LPS-Stimulated RAW264.7 Cells" Molecules 22, no. 10: 1583. https://doi.org/10.3390/molecules22101583