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Molecules 2017, 22(1), 67; doi:10.3390/molecules22010067

Preparation, Phytochemical Investigation, and Safety Evaluation of Chlorogenic Acid Products from Eupatorium adenophorum

Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
University of Chinese Academy of Sciences, Beijing 100049, China
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 6 December 2016 / Revised: 27 December 2016 / Accepted: 29 December 2016 / Published: 31 December 2016
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [813 KB, uploaded 31 December 2016]   |  


Eupatorium adenophorum is widely distributed throughout the world’s tropical and temperate regions. It has become a harmful weed of crops and natural environments. Its leaves contain bioactive compounds such as chlorogenic acid and may be used as feed additives. In this study, chlorogenic acid was extracted and separated from leaves of E. adenophorum. Three chlorogenic acid products were prepared with different purities of 6.11%, 22.17%, and 96.03%. Phytochemical analysis demonstrated that the main toxins of sesquiterpenes were almost completely removed in sample preparation procedure. The three products were evaluated for safety via in vitro and in vivo toxicological studies. All the products exhibited no cytotoxic effects at a dose of 400 μg/mL in an in vitro cell viability assay. When administered in vivo at a single dose up to 1.5 g/kg bw, all three products caused no signs or symptoms of toxicity in mice. These results encourage further exploration of extracts from E. adenophorum in feed additive application. View Full-Text
Keywords: Eupatorium adenophorum; chlorogenic acid; sesquiterpenes; safety; toxicity Eupatorium adenophorum; chlorogenic acid; sesquiterpenes; safety; toxicity

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Liu, B.; Cao, L.; Zhang, L.; Yuan, X.; Zhao, B. Preparation, Phytochemical Investigation, and Safety Evaluation of Chlorogenic Acid Products from Eupatorium adenophorum. Molecules 2017, 22, 67.

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