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Molecules 2017, 22(1), 166; doi:10.3390/molecules22010166

Characterization of Collagen Peptides in Elaphuri Davidiani Cornu Aqueous Extract with Proliferative Activity on Osteoblasts Using Nano-Liquid Chromatography in Tandem with Orbitrap Mass Spectrometry

1
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China
2
Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China
3
Jiangsu Dafeng Milu National Nature Reserve, Dafeng 224136, China
4
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 3 October 2016 / Revised: 26 December 2016 / Accepted: 12 January 2017 / Published: 20 January 2017
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Abstract

First documented in Shennong Bencao Jing (about 200 B.C.–200 A.D.), Elaphuri Davidiani Cornu (EDC) has been recorded for its effects in strengthening bones and balancing other aspects of overall health for approximately 2000 years. In the present study, our aim was to investigate which are the components of the active EDC fraction by a peptidomic strategy. We explored the extent to which EDC increases the proliferation of osteoblasts by measuring the elevations in osteonectin and type I collagen mRNA levels and characterized it using nano-flow liquid chromatography in tandem with orbitrap mass spectrometry. In total, 272 peptide sequences from collagens were determined. “Hot regions” in parent proteins determined by peptide heat maps which indicated that amino acid sequences in the regions might undergo proteolysis easily and generate peptides. Among the identified peptides, 90.2% were hydrophilic, and the molecular weight of 97.1% of identified peptides was lower than 2000 Da. According to these results, EDC collagen-derived peptides were easily analyzed and identified. Moreover, this methodology is feasible to characterize the active peptides matrices originated from collagen hydrolysates or some other animal horn- derived TCMs. View Full-Text
Keywords: collagen peptide; Elaphuri Davidiani Cornu; nano-liquid chromatography-mass spectrum detection; osteoblast collagen peptide; Elaphuri Davidiani Cornu; nano-liquid chromatography-mass spectrum detection; osteoblast
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MDPI and ACS Style

Zhai, Y.; Zhu, Z.; Zhu, Y.; Qian, D.; Liu, R.; Peng, Y.; Ding, Y.; Ouyang, Z.; Duan, J.-A. Characterization of Collagen Peptides in Elaphuri Davidiani Cornu Aqueous Extract with Proliferative Activity on Osteoblasts Using Nano-Liquid Chromatography in Tandem with Orbitrap Mass Spectrometry. Molecules 2017, 22, 166.

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