Next Article in Journal
Novel Amino-Pyridine Functionalized Chitosan Quaternary Ammonium Derivatives: Design, Synthesis, and Antioxidant Activity
Previous Article in Journal
Ultrafast Electronic Deactivation Dynamics of Xanthosine Monophosphate
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Molecules 2017, 22(1), 157; doi:10.3390/molecules22010157

Synthetic Assembly of Mannose Moieties Using Polymer Chemistry and the Biological Evaluation of Its Interaction towards Concanavalin A

Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 12 December 2016 / Revised: 10 January 2017 / Accepted: 12 January 2017 / Published: 18 January 2017
(This article belongs to the Section Bioorganic Chemistry)
View Full-Text   |   Download PDF [1435 KB, uploaded 18 January 2017]   |  

Abstract

Protein–carbohydrate interactions exhibit myriad intracellular recognition events, so understanding and investigating their specific interaction with high selectivity and strength are of crucial importance. In order to examine the effect of multivalent binding on the specificity of protein–carbohydrate interactions, we synthesized mannose glycosides as a novel type of glycosylated monomer and glycopolymers of polyacrylamide derivatives with α-mannose (α-Man) by radical polymerization and monitored their strength of interaction with concanavalin A (Con A) by surface plasmon resonance (SPR) detection. In a quantitative test using the Con A-immobilized sensor surface, the kinetic affinity for the synthesized polymers, 8a (KD = 3.3 × 10−6 M) and 8b (KD = 5.3 × 10−5 M), were concentration-dependent, showing strong, specific molecular recognition abilities with lectin. Our study showed the enhancement in recognition specificity for multivalent saccharides, which is often mediated by cell surface carbohydrate-binding proteins that exhibit weak affinity and broad specificity for the individual ligands. View Full-Text
Keywords: mannose; glycopolymers; concanavalin A; surface plasmon resonance; radical polymerization; kinetic affinity mannose; glycopolymers; concanavalin A; surface plasmon resonance; radical polymerization; kinetic affinity
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Diwan, D.; Shinkai, K.; Tetsuka, T.; Cao, B.; Arai, H.; Koyama, T.; Hatano, K.; Matsuoka, K. Synthetic Assembly of Mannose Moieties Using Polymer Chemistry and the Biological Evaluation of Its Interaction towards Concanavalin A. Molecules 2017, 22, 157.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top