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Molecules 2016, 21(9), 1221; doi:10.3390/molecules21091221

Suitable DNA Barcoding for Identification and Supervision of Piper kadsura in Chinese Medicine Markets

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1
College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
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Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China
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Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 20 July 2016 / Accepted: 7 September 2016 / Published: 12 September 2016
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [2703 KB, uploaded 12 September 2016]   |  

Abstract

Piper kadsura is a vine-like medicinal plant which is widely used in clinical treatment. However, P. kadsura is often substituted by other materials in the markets, thereby causing health risks. In this study, 38 P. kadsura samples and eight sequences from GenBank, including a closely-related species and common adulterants were collected. This study aimed to identify an effective DNA barcode from four popular DNA loci for P. kadsura authentication. The success rates of PCR amplification, sequencing, and sequence acquisition of matK were 10.5%, 75%, and 7.9%, respectively; for rbcL they were 89.5%, 8.8%, and 7.9%, respectively; ITS2 rates were 86.8%, 3.0%, and 2.6%, respectively, while for psbA-trnH they were all 100%, which is much higher than for the other three loci. The sequences were aligned using Muscle, genetic distances were computed using MEGA 5.2.2, and barcoding gap was performed using TAXON DNA. Phylogenetic analysis showed that psbA-trnH could clearly distinguish P. kadsura from its closely related species and the common adulterant. psbA-trnH was then used to evaluate the fake proportions of P. kadsura. Results showed that 18.4% of P. kadsura samples were fake, indicating that adulterant species exist in the Chinese markets. Two-dimensional DNA barcoding imaging of P. kadsura was conducted, which was beneficial to the management of P. kadsura. We conclude that the psbA-trnH region is a powerful tool for P. kadsura identification and supervision in the current medicine markets. View Full-Text
Keywords: Piper kadsura; DNA barcoding; psbA-trnH; medicine market supervision Piper kadsura; DNA barcoding; psbA-trnH; medicine market supervision
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Yu, N.; Gu, H.; Wei, Y.; Zhu, N.; Wang, Y.; Zhang, H.; Zhu, Y.; Zhang, X.; Ma, C.; Sun, A. Suitable DNA Barcoding for Identification and Supervision of Piper kadsura in Chinese Medicine Markets. Molecules 2016, 21, 1221.

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