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Molecules 2016, 21(9), 1208; doi:10.3390/molecules21091208

Impact of Biohybrid Magnetite Nanoparticles and Moroccan Propolis on Adherence of Methicillin Resistant Strains of Staphylococcus aureus

1
Laboratory of Physiology-Pharmacology-Environmental Health, Faculty of Sciences Dhar El Mehraz, BP 1796 Atlas, University Sidi Mohamed Ben Abdallah, Fez 30 000, Morocco
2
Department of Chemistry and Pharmacy, Faculty of Science and Technology, University of Algarve, Campus de Gambelas, MeditBio, Faro 8005-139, Portugal
3
Institute of Organic Chemistry with Centre of Phytochemistry, Acad. G. Bonchev strl. bl. 9, Sofia 1113, Bulgaria
4
Algarve Chemistry Research Centre(CIQA) and Department of Chemistry and Pharmacy, Faculty of Science and Technology, University of Algarve, Faro 8005-139, Portugal
5
CQE, Centro de Química Estrutural, Instituto Superior Técnico, University of Lisbon, Av. Rovisco Pais, Lisboa 1049-001, Portugal
6
Department of Physics and CeFEMA, Faculty of Science and Technology, University of Algarve, Campus de Gambelas, Faro 8005-139, Portugal
7
Department of Biological Sciences and Bioengineering, Faculty of Science and Technology, University of Algarve, Center for Biomedical Research, Faro 8005-139, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 21 July 2016 / Revised: 28 August 2016 / Accepted: 2 September 2016 / Published: 9 September 2016
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Abstract

Biofilm bacteria are more resistant to antibiotics than planktonic cells. Propolis possesses antimicrobial activity. Generally, nanoparticles containing heavy metals possess antimicrobial and antibiofilm properties. In this study, the ability of adherence of Methicillin Resistant Strains of Staphylococcus aureus (MRSA) to catheters treated with magnetite nanoparticles (MNPs), produced by three methods and functionalized with oleic acid and a hydro-alcoholic extract of propolis from Morocco, was evaluated. The chemical composition of propolis was established by gas chromatography mass spectrometry (GC-MS), and the fabricated nanostructures characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), Mossbauer spectroscopy and Fourrier transform infrared spectroscopy (FTIR). The capacity for impairing biofilm formation was dependent on the strain, as well as on the mode of production of MNPs. The co-precipitation method of MNPs fabrication using Fe3+ and Na2SO3 solution and functionalized with oleic acid and propolis was the most effective in the impairment of adherence of all MRSA strains to catheters (p < 0.001). The adherence of the strain MRSA16 was also significantly lower (p < 0.001) when the catheters were treated with the hybrid MNPs with oleic acid produced by a hydrothermal method. The anti-MRSA observed can be attributed to the presence of benzyl caffeate, pinocembrin, galangin, and isocupressic acid in propolis extract, along with MNPs. However, for MRSA16, the impairment of its adherence on catheters may only be attributed to the hybrid MNPs with oleic acid, since very small amount, if any at all of propolis compounds were added to the MNPs. View Full-Text
Keywords: co-precipitation; hydrothermal; functionalization; propolis; flavonoids; diterpenes co-precipitation; hydrothermal; functionalization; propolis; flavonoids; diterpenes
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MDPI and ACS Style

El-Guendouz, S.; Aazza, S.; Lyoussi, B.; Bankova, V.; Lourenço, J.P.; Costa, A.M.R.; Mariano, J.F.; Miguel, M.G.; Faleiro, M.L. Impact of Biohybrid Magnetite Nanoparticles and Moroccan Propolis on Adherence of Methicillin Resistant Strains of Staphylococcus aureus. Molecules 2016, 21, 1208.

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